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The results showed that the fluorescence quenching mechanism was static quenching by the formation of an HSA\u2013FPZ complex. Entropy change (\u0394<jats:italic>S<\/jats:italic><jats:sup>0<\/jats:sup>) and enthalpy change (\u0394<jats:italic>H<\/jats:italic><jats:sup>0<\/jats:sup>) values were 68.42\u00a0J\/(mol<jats:styled-content>\u22c5<\/jats:styled-content>K) and \u22124.637\u00a0kJ\/mol, respectively, which indicated that hydrophobic interactions and hydrogen bonds played major roles in the acting forces. The interaction process was spontaneous because the Gibbs free energy change (\u0394<jats:italic>G<\/jats:italic><jats:sup>0<\/jats:sup>) values were negative. The results of competitive experiments demonstrated that FPZ was mainly located within HSA site I (sub\u2010domain IIA). Molecular docking results were in agreement with the experimental conclusions of the thermodynamic parameters and competition experiments. Competitive binding to HSA between flavonoids and FPZ decreased the association constants and increased the binding distances of FPZ binding to HSA. The results of absorption, synchronous fluorescence, three\u2010dimensional fluorescence, and CD spectra showed that the binding of FPZ to HSA caused conformational changes in HSA and simultaneous effects of FPZ and flavonoids induced further HSA conformational changes.<\/jats:p>","DOI":"10.1002\/bio.3291","type":"journal-article","created":{"date-parts":[[2017,4,5]],"date-time":"2017-04-05T12:10:52Z","timestamp":1491394252000},"page":"1056-1065","source":"Crossref","is-referenced-by-count":17,"title":["Binding of fluphenazine with human serum albumin in the presence of rutin and quercetin: An evaluation of food\u2010drug interaction by spectroscopic techniques"],"prefix":"10.1002","volume":"32","author":[{"given":"Jiao\u2010Jiao","family":"Jing","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences Liaoning University  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Liaoning University  Shenyang China"},{"name":"Liaoning Provincial Engineering Research Center for Natural Products Pharmaceutical  Shenyang China"},{"name":"Liaoning Provincial Key Laboratory of New Drug R&amp;D  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0048-9232","authenticated-orcid":false,"given":"Xin","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Liaoning University  Shenyang China"},{"name":"Liaoning Provincial Engineering Research Center for Natural Products Pharmaceutical  Shenyang China"},{"name":"Liaoning Provincial Key Laboratory of New Drug R&amp;D  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Liaoning University  Shenyang China"},{"name":"Liaoning Provincial Engineering Research Center for Natural Products Pharmaceutical  Shenyang China"},{"name":"Liaoning Provincial Key Laboratory of New Drug R&amp;D  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling\u2010Ling","family":"He","sequence":"additional","affiliation":[{"name":"College of Applied Chemistry Shenyang University of Chemical Technology  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue\u2010Yuan","family":"Guo","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Liaoning University  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming\u2010Ling","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Liaoning University  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian\u2010Yu","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Liaoning University  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Gao","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Liaoning University  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo\u2010Yang","family":"Dong","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Liaoning University  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,4,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-5335-9_13"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.4103\/0975-7406.137256"},{"key":"e_1_2_7_4_1","first-page":"22","volume":"2","author":"Lakhanpal P.","year":"2007","journal-title":"Internet J. 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