{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T12:33:55Z","timestamp":1784723635301,"version":"3.55.0"},"reference-count":20,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2017,4,21]],"date-time":"2017-04-21T00:00:00Z","timestamp":1492732800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Research Fund of Istanbul University","award":["55961"],"award-info":[{"award-number":["55961"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A new method based on fluorescence derivatization with 5\u2010(dimethylamino) naphthalene\u20101\u2010sulfonyl chloride (dansyl chloride) was developed for the quantitative determination of galantamine in human plasma and urine using high\u2010performance liquid chromatography. The reaction between galantamine and dansyl chloride was optimally realized in 30\u00a0min at room temperature and pH\u00a010.5, with a reagent to galantamine molar ratio of 2.13. The derivative was extracted with dichloromethane, and the extract was dried under a nitrogen stream and dissolved in the mobile phase. Chromatographic analysis was performed with an Inertsil C<jats:sub>18<\/jats:sub> column and a mobile phase comprising 40% acetonitrile and 60% 10\u00a0mM <jats:italic>o<\/jats:italic>\u2010phosphoric acid, 1.2\u00a0ml\/min. The injection volume was 20\u00a0\u03bcl. The derivatives were detected with a fluorescence detector (excitation 375\u00a0nm\/emission 537\u00a0nm). The retention time for the dansyl derivative of galantamine was 16.8\u00a0min. Linearity was observed between 125 and 2000\u00a0ng\/ml in water, urine and plasma. The limit of detection and limit of quantification for the developed method were 6.27\u201370.99 and 18.81\u2013212.97\u00a0ng\/ml, respectively. Per cent recovery was calculated as 95.15 for urine and 95.78 for plasma. Interday repeatability values for urine and plasma samples (<jats:italic>n<\/jats:italic>\u00a0=\u00a06) at three different concentrations were calculated as a per cent relative standard deviation of 0.24\u20130.59 and 0.35\u20130.56. The corresponding per cent relative standard deviation values for intraday repeatability were 0.13\u20130.51 and 0.04\u20130.15, respectively.<\/jats:p>","DOI":"10.1002\/bio.3301","type":"journal-article","created":{"date-parts":[[2017,4,21]],"date-time":"2017-04-21T09:53:56Z","timestamp":1492768436000},"page":"1145-1149","source":"Crossref","is-referenced-by-count":9,"title":["Highly sensitive HPLC method for the determination of galantamine in human plasma and urine through derivatization with dansyl chloride using fluorescence detector"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5011-8344","authenticated-orcid":false,"given":"Elif","family":"\u00d6zdemir","sequence":"first","affiliation":[{"name":"Department of Analytical Chemistry Istanbul Yeni Yuzyil University  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sevgi","family":"Tatar Ulu","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry Istanbul University  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,4,21]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"Rx Media Pharma2015."},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/clpt.1991.159"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.4103\/0975-7406.120079"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2010.04.043"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.18579\/jpcrkc\/2013\/12\/1\/79129"},{"key":"e_1_2_7_7_1","first-page":"1689","volume":"64","author":"Tsvetkova D.","year":"2011","journal-title":"C. 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