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As known, the <jats:italic>o<\/jats:italic>\u2010phenanthroline (<jats:italic>o<\/jats:italic>\u2010phen) has inner fluorescence, though when reacted with zinc ions to form the <jats:italic>o<\/jats:italic>\u2010phen\u2013Zn<jats:sup>2+<\/jats:sup> complex the fluorescence intensity was enhanced effectively, while upon addition of TM into the <jats:italic>o<\/jats:italic>\u2010phen\u2013Zn<jats:sup>2+<\/jats:sup> complex solution, the intensity of the system was gently quenched, which was termed the \u2018turn\u2010on\u2013off\u2019 probe. Notably, the method possessed highly selective, sensitive determination for TM with a detection limit of 0.25\u00a0\u03bcmol\u00a0L<jats:sup>\u22121<\/jats:sup> and the reduced fluorescence intensity was proportional to the concentration of TM in the range 0.84\u201380.0\u00a0\u03bcmol\u00a0L<jats:sup>\u22121<\/jats:sup>. Besides, the proposed mechanism was also investigated through exploring the Fourier transform infrared (FT\u2010IR), nuclear magnetic resonance (NMR) spectroscopy. Furthermore, this manner was successfully applied into practical samples for TM detection with satisfactory results.<\/jats:p>","DOI":"10.1002\/bio.3519","type":"journal-article","created":{"date-parts":[[2018,7,13]],"date-time":"2018-07-13T08:25:57Z","timestamp":1531470357000},"page":"1128-1135","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Construction of a novel turn\u2010on\u2013off fluorescence sensor used for highly selective detection of thiamine via its quenching effect on <i>o<\/i>\u2010phen\u2010Zn<sup>2+<\/sup> complex"],"prefix":"10.1002","volume":"33","author":[{"given":"Hui","family":"Zhang","sequence":"first","affiliation":[{"name":"Key Laboratory of Luminescent and Real\u2010time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering Southwest University  Chongqing P. R. 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