{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T18:27:41Z","timestamp":1760898461805,"version":"build-2065373602"},"reference-count":48,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,9,27]],"date-time":"2018-09-27T00:00:00Z","timestamp":1538006400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010221","name":"Higher Education Commision, Pakistan","doi-asserted-by":"publisher","award":["20\u20103968"],"award-info":[{"award-number":["20\u20103968"]}],"id":[{"id":"10.13039\/501100010221","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The simultaneous assay of carboxymethylflavin (CMF), an intermediate in the photolysis of riboflavin, and its hydrolytic side\u2010chain cleavage products, lumichrome (LC) (acid solution) and LC and lumiflavin (LF) as well as isoalloxazine ring cleavage products, 1,2\u2010dihydro\u20101\u2010methyl\u20102\u2010keto\u20103\u2010quinoxaline carboxylic acid (KA) and 1,2,3,4\u2010tetrahydro\u20101\u2010methyl\u20102,3\u2010dioxo\u2010quinoxaline (DQ) (alkaline solution) has been carried out by a multicomponent spectrofluorimetric method. The method is based on the adjustment of pH of the degraded solutions to 2.0 and extraction of LC and LF with chloroform. The chloroform extract is evaporated to dryness under reduced pressure, the residue dissolved in pH\u00a06.5 citro\u2010phosphate buffer and LC and LF determined at their fluorescence maxima at 478 and 530\u00a0nm, respectively. The pH of the aqueous phase is re\u2010adjusted to 6.5 and the solution used for the determination of CMF, KA and DQ at the wavelengths of 530, 443 and 420\u00a0nm, respectively. The proposed method has been validated according to ICH guidelines. The calibration curves for CMF and its hydrolytic products are linear in the concentration range of 0.5\u20135.0\u00a0\u00d7\u00a010<jats:sup>\u22126<\/jats:sup>\u00a0M. The mean recovery ranges from 99.0\u2013102.0% with relative standard deviation (RSD) of 0.19\u20130.99%. The limit of detection (LOD) and the limit of quantification (LOQ) are in the range of 1.17\u20131.78\u00a0\u00d7\u00a010<jats:sup>\u22127<\/jats:sup>\u00a0M and 3.55\u20135.40\u00a0\u00d7\u00a010<jats:sup>\u22127<\/jats:sup>\u00a0M, respectively. The uniformity of molar balance of CMF and degradation products during hydrolytic reactions indicates the accuracy of the proposed method for the spectrofluorimetric assay of the compounds. It has been applied to study the kinetics of hydrolytic reactions of CMF.<\/jats:p>","DOI":"10.1002\/bio.3549","type":"journal-article","created":{"date-parts":[[2018,9,27]],"date-time":"2018-09-27T04:31:34Z","timestamp":1538022694000},"page":"1314-1325","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Multicomponent spectrofluorimetric method for the assay of carboxymethylflavin and its hydrolytic products: kinetic applications"],"prefix":"10.1002","volume":"33","author":[{"given":"Tania","family":"Mirza","sequence":"first","affiliation":[{"name":"Baqai Institute of Pharmaceutical Sciences, Baqai Medical University  Super Highway, Gadap Road Karachi Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3822-7588","authenticated-orcid":false,"given":"Zubair","family":"Anwar","sequence":"additional","affiliation":[{"name":"Baqai Institute of Pharmaceutical Sciences, Baqai Medical University  Super Highway, Gadap Road Karachi Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Ahsan","family":"Ejaz","sequence":"additional","affiliation":[{"name":"Baqai Institute of Pharmaceutical Sciences, Baqai Medical University  Super Highway, Gadap Road Karachi Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sofia","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Baqai Institute of Pharmaceutical Sciences, Baqai Medical University  Super Highway, Gadap Road Karachi Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6333-8178","authenticated-orcid":false,"given":"Muhammad Ali","family":"Sheraz","sequence":"additional","affiliation":[{"name":"Baqai Institute of Pharmaceutical Sciences, Baqai Medical University  Super Highway, Gadap Road Karachi Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iqbal","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Baqai Institute of Pharmaceutical Sciences, Baqai Medical University  Super Highway, Gadap Road Karachi Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,9,27]]},"reference":[{"volume-title":"Fluorescence Assay in Biology and Medicine","year":"1969","author":"Horecker B.","key":"e_1_2_6_2_1"},{"volume-title":"Fluorimetric Techniques in Clinical Chemistry","year":"1973","author":"Elevtich F. 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