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Compound <jats:bold>L<\/jats:bold> displayed fluorescence quenching response only towards copper(II) ions (Cu<jats:sup>2+<\/jats:sup>) in acetonitrile solution with high selectivity and sensitivity. However, compound <jats:bold>A<\/jats:bold> presented \u2018on\u2013off\u2019 fluorescence response towards a wide range of metal ions to different degrees and did not have selectivity. Furthermore, compound <jats:bold>L<\/jats:bold> formed a 1:1 complex with Cu<jats:sup>2+<\/jats:sup> and the binding constant between sensor <jats:bold>L<\/jats:bold> and Cu<jats:sup>2+<\/jats:sup> was high at 6.02\u00a0\u00d7\u00a010<jats:sup>4<\/jats:sup>\u00a0M<jats:sup>\u22121<\/jats:sup>. Job's plot, mass spectra, IR spectra, <jats:sup>1<\/jats:sup>H\u2010NMR titration and density functional theory (DFT) calculations demonstrated the formation of a 1:1 complex between <jats:bold>L<\/jats:bold> and Cu<jats:sup>2+<\/jats:sup>. Chemosensor <jats:bold>L<\/jats:bold> displayed a low limit of detection (3.05\u00a0\u00d7\u00a010<jats:sup>\u22126<\/jats:sup>\u00a0M) and fast response time (15\u00a0s) to Cu<jats:sup>2+<\/jats:sup>. The Stern\u2013Volmer analysis illustrated that the fluorescence quenching agreed with the static quenching mode. In addition, the obvious difference of <jats:bold>L<\/jats:bold> within HepG2 cells in the presence and absence of Cu<jats:sup>2+<\/jats:sup> indicated <jats:bold>L<\/jats:bold> had the recognition capability for Cu<jats:sup>2+<\/jats:sup> in living cells.<\/jats:p>","DOI":"10.1002\/bio.3586","type":"journal-article","created":{"date-parts":[[2019,1,10]],"date-time":"2019-01-10T01:56:54Z","timestamp":1547085414000},"page":"153-161","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["A new benzimidazole\u2010based selective and sensitive \u2018on\u2013off\u2019 fluorescence chemosensor for Cu<sup>2+<\/sup> ions and application in cellular bioimaging"],"prefix":"10.1002","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2616-0497","authenticated-orcid":false,"given":"Yi","family":"He","sequence":"first","affiliation":[{"name":"College of Chemistry Jilin University  Changchun P. R. 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