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Analysis of <jats:bold>Mix I<\/jats:bold> is based on the measurement of the peak amplitudes (D<jats:sup>1<\/jats:sup>) of synchronous fluorescence intensities at 265.1 nm for DIP and 260 nm for IBU. The relationship between the concentration and the amplitude of the first\u2010derivative synchronous fluorescence spectra showed good linearity over the concentration ranges 0.50\u201310.00 \u03bcg ml<jats:sup>\u22121<\/jats:sup> and 0.50\u20137.90 \u03bcg ml<jats:sup>\u22121<\/jats:sup> for DIP and IBU, respectively. Analysis of <jats:bold>Mix II<\/jats:bold> was based on measurement of the peak amplitude (D<jats:sup>1<\/jats:sup>) synchronous fluorescence intensities at 230 nm for DIP and at 253.9 nm for PHE. Moreover, for <jats:bold>Mix II,<\/jats:bold> the peak amplitude (D<jats:sup>2<\/jats:sup>) synchronous fluorescence intensities were measured at 227.9 nm for DIP and at 264.9 nm for PHE. Calibration plots were rectilinear over the concentration range 0.30\u20133.50 \u03bcg ml<jats:sup>\u22121<\/jats:sup> and 0.03\u20130.75 \u03bcg ml<jats:sup>\u22121<\/jats:sup> for DIP and PHE, respectively. The proposed methods were successfully applied to determine the studied compounds in pure form and in pharmaceutical preparations.<\/jats:p>","DOI":"10.1002\/bio.3750","type":"journal-article","created":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T06:18:27Z","timestamp":1578291507000},"page":"550-560","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Synchronous spectrofluorometric methods for simultaneous determination of diphenhydramine and ibuprofen or phenylephrine in combined pharmaceutical preparations"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4479-7723","authenticated-orcid":false,"given":"Aya","family":"Saad Radwan","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Tanta University  Egypt"},{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy Horus University \u2013 Egypt  New Damietta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4429-6504","authenticated-orcid":false,"given":"Mohamed M.","family":"Salim","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Mansoura University  Mansoura Egypt"},{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy Horus University \u2013 Egypt  New Damietta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2102-1081","authenticated-orcid":false,"given":"Sherin F.","family":"Hammad","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Tanta University  Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,1,6]]},"reference":[{"key":"e_1_2_6_2_1","volume-title":"Martindale: The complete drug reference","author":"Sweetman S. 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