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Here we designed a bioluminescent turn\u2010on probe, HNO\u20108, that could be used to visualize HNO without the need for excitation light. HNO\u20108 was prepared by caging 2\u2010hydroxyethyl luciferin with a triphenylphosphine unit, in which 2\u2010hydroxyethyl luciferin as a novel substrate of firefly luciferase was characterized by stronger and more sustained bioluminescent signals than the most popular substrates of <jats:sc>d<\/jats:sc>\u2010luciferin and 6\u2032\u2010aminoluciferin. <jats:italic>In vitro<\/jats:italic> experiments showed that HNO\u20108 could selectively respond to HNO generated from Angeli's salt(AS) in the range 1\u201350 \u03bcM, with a limit of detection of 0.196 \u03bcM. The probe was successfully applied for visualizing HNO in luciferase\u2010transfected Huh7 cancer cells. We envision that HNO\u20108 could be used as a powerful bioluminescent sensor for researching HNO biological roles.<\/jats:p>","DOI":"10.1002\/bio.3902","type":"journal-article","created":{"date-parts":[[2020,6,15]],"date-time":"2020-06-15T15:38:50Z","timestamp":1592235530000},"page":"1384-1390","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A caged 2\u2010hydroxyethyl luciferin for bioluminescence imaging of nitroxyl in living cells"],"prefix":"10.1002","volume":"35","author":[{"given":"Yong","family":"Ju","sequence":"first","affiliation":[{"name":"School of Chemical and Environmental Engineering Shanghai Institute of Technology  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anni","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy  Fudan University, 826 Zhangheng Road Shanghai 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