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The determination in biological fluids of a co\u2010administered antidepressant drug and a sexual stimulation drug is a very critical and important step for psychotic and ischaemic heart disease patients, especially in cases of emergency and this requires therapeutic drug monitoring. A sensitive, efficient and rapid assay was selected satisfactorily and applied for simultaneous determination of citalopram and tadalafil either in their pure forms, in tablet dosage forms or in spiked human plasma. There was a large overlap for both drugs, forming the broad band found in conventional fluorescence spectra and their related synchronous fluorescence intensity. Therefore, the development of a highly sensitive second derivative synchronous fluorescence method was demonstrated that removed this overlap. The proposed method depended on measuring the amplitudes of the second derivative of synchronous fluorescence intensity at suitable wavelengths of 301 nm and 367 nm for citalopram and tadalafil at \u0394\u03bb = 60 nm, respectively. Box\u2013Behnken design as a response surface methodology was used to fit models and create an optimization process encompassing a set of factors and resulting in an optimum response value specifically designed for this method. Under optimum conditions, the linear dynamic ranges for citalopram and tadalafil estimation were 20\u2013900 and 5\u2013400 ng ml<jats:sup>\u22121<\/jats:sup> with detection limits of 5.40 and 1.43 ng ml<jats:sup>\u22121<\/jats:sup>, respectively.<\/jats:p>","DOI":"10.1002\/bio.3913","type":"journal-article","created":{"date-parts":[[2020,7,17]],"date-time":"2020-07-17T13:40:52Z","timestamp":1594993252000},"page":"57-65","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Simultaneous determination of citalopram and tadalafil by the second derivative synchronous fluorescence method in biological fluids; application of Box\u2013Behnken optimization design"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4111-9716","authenticated-orcid":false,"given":"Ahmed M.","family":"Abdel\u2010Raoof","sequence":"first","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Al\u2010Azhar University  Nasr City Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9483-3841","authenticated-orcid":false,"given":"Mohamed A.","family":"Hasan","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Al\u2010Azhar University  Nasr City Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed W.","family":"Madkour","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Al\u2010Azhar University  Nasr City Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashraf","family":"Abdel\u2010Fattah","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Al\u2010Azhar University  Nasr City Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3246-418X","authenticated-orcid":false,"given":"Maya S.","family":"Eissa","sequence":"additional","affiliation":[{"name":"Pharmaceutical Chemistry Department, Faculty of Pharmacy Egyptian Russian University  Badr City Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-199141030-00008"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1176\/appi.ajp.161.6.1079"},{"key":"e_1_2_9_4_1","volume-title":"The Merck Index","author":"Budavari S.","year":"2007"},{"key":"e_1_2_9_5_1","first-page":"65","volume":"89","author":"El\u2010Gindy A.","year":"2006","journal-title":"J. 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