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The suggested method relies on the construction of a derivative with strong fluorescence called dihydropyridine derivative. This derivative resulted from the interaction of the primary amino group in the studied drug and acetylacetone\/formaldehyde in McIlvaine buffer (pH 5). The fluorescent dihydropyridine derivative was measured at 470 nm. Influences of experimental variables namely pH, reagent concentration and temperature were examined and optimized. The calibration curve showed linearity over the range of 0.15\u20131.25 \u03bcg ml<jats:sup>\u22121<\/jats:sup> of milnacipran with an R<jats:sup>2<\/jats:sup> value of 0.9998. The detection limit was 0.02 \u03bcg ml<jats:sup>\u22121<\/jats:sup> and the determination limit was 0.07 \u03bcg ml<jats:sup>\u22121<\/jats:sup>. The developed procedure was successfully used in the assay of the studied drug in Avermilan\u00ae tablets with excellent selectivity. In addition, the reaction was applied to estimate the drug in spiked human plasma and urine with mean percentage recoveries of 100.04 \u00b1 1.61 and 99.78 \u00b1 0.81% for urine and plasma, respectively.<\/jats:p>","DOI":"10.1002\/bio.3921","type":"journal-article","created":{"date-parts":[[2020,7,24]],"date-time":"2020-07-24T12:40:33Z","timestamp":1595594433000},"page":"73-78","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Use of Hantzsch reaction for quantitation of milnacipran as a magic treatment for fibromyalgia syndrome in human plasma and urine"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3703-7221","authenticated-orcid":false,"given":"Islam M.","family":"Mostafa","sequence":"first","affiliation":[{"name":"Analytical Chemistry Department, Faculty of Pharmacy Minia University  Minia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmoud A.","family":"Omar","sequence":"additional","affiliation":[{"name":"Analytical Chemistry Department, Faculty of Pharmacy Minia University  Minia Egypt"},{"name":"Pharmacognosy and Pharmaceutical Chemistry Department, College of Pharmacy Taibah University  Medinah Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dalia M.","family":"Nagy","sequence":"additional","affiliation":[{"name":"Analytical Chemistry Department, Faculty of Pharmacy Minia University  Minia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5357-1761","authenticated-orcid":false,"given":"Sayed M.","family":"Derayea","sequence":"additional","affiliation":[{"name":"Analytical Chemistry Department, Faculty of Pharmacy Minia University  Minia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,9]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"637","volume":"7","author":"Leo R. 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