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The Cys\u2010CuNCs were successfully synthesized and characterized using UV\u2013visible absorption, emission, Fourier\u2010transform infrared (FT\u2010IR) spectroscopy, fluorescence lifetime, high resolution transmission electron microscopy (HR\u2010TEM) and zeta potential measurements. Cys\u2010CuNCs exhibited bluish\u2010green luminescence under UV light with characteristic emission maxima at 486 nm. Cys\u2010CuNCs was successfully exploited as fluorescent probes for the detection of Rut\/Que. The addition of increasing concentrations of Rut\/Que led to a gradual decrease in the emission intensity of Cys\u2010CuNCs. The decrease in Cys\u2010CuNC emission intensities were attributed to the strong IFE and static quenching mechanism. The calculated limit of detection values for Rut and Que were as low as 0.021 \u03bcM and 0.035 \u03bcM, respectively. The Cys\u2010CuNCs sensing probe exhibited excellent selectivity in the presence of other potential interfering compounds. Furthermore, the present method was successfully applied to the analysis of both Rut and Que in biological samples.<\/jats:p>","DOI":"10.1002\/bio.3945","type":"journal-article","created":{"date-parts":[[2020,9,25]],"date-time":"2020-09-25T07:18:52Z","timestamp":1601018332000},"page":"326-335","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Water\u2010soluble luminescent copper nanoclusters as a fluorescent quenching probe for the detection of rutin and quercetin based on the inner filter effect"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4683-7626","authenticated-orcid":false,"given":"Thangarasu","family":"Sasikumar","sequence":"first","affiliation":[{"name":"Department of Chemistry Bharathiar University  Coimbatore Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malaichamy","family":"Ilanchelian","sequence":"additional","affiliation":[{"name":"Department of Chemistry Bharathiar University  Coimbatore Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,10,20]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-827X(01)01111-9"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0753-3322(97)88045-6"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00604-007-0888-7"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11051-011-0675-6"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7AY01480E"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5AY00353A"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2006-957866"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0271-5317(01)00398-0"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2670(00)01099-0"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0271-5317(96)00032-2"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2017.04.006"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/elan.201500490"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2017.11.059"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf101580j"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2013.10.036"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12161-012-9551-y"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2004.06.077"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jelechem.2017.01.001"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2014.09.027"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2012.08.040"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2006.06.004"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2008.07.011"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf010354p"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2003.07.007"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2014.01.009"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2015.12.013"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2014.07.019"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1039\/c1nr00006c"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nantod.2011.06.004"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpcc.5b08123"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpclett.5b00378"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1039\/C3CC47771A"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201204052"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/am505799q"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac202610e"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1039\/C3NR04835G"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200907256"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/nn3002455"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja109303z"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.bioconjchem.5b00003"},{"key":"e_1_2_6_42_1","first-page":"1","volume":"25","author":"Ma J. 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