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A luciferase\u2010like enzyme, previously cloned from the Malpighian tubules of the non\u2010luminescent <jats:italic>Zophobas morio<\/jats:italic> mealworm, displays thioesterification activity with a wide range of carboxylic substrates, and produces weak red luminescence in the presence of ATP and firefly <jats:sc>d<\/jats:sc>\u2010luciferin, a xenobiotic for this organism. To better investigate the function of this enzyme in carboxylic xenobiotic detoxification, we analyzed the inhibitory effect of different xenobiotic carboxylic acids on the luminescence activity of this enzyme, including environmental pollutants and pharmaceutical compounds. Noteworthy, the anti\u2010inflammatory drug diclofenac severely inhibited this luciferase\u2010like enzyme luminescence activity, both in <jats:italic>in vitro<\/jats:italic> (IC<jats:sub>50<\/jats:sub> 20 \u03bcM) and <jats:italic>in vivo<\/jats:italic> in bacterial cells assays, when compared with other beetle luciferases. Similar results were obtained with its brighter I327S mutant. Kinetic analysis of diclofenac's effect on luminescence activity indicated mixed\u2010type inhibition for both ATP and <jats:sc>d<\/jats:sc>\u2010luciferin. Modelling studies showed five potential binding sites for diclofenac, including the coenzyme A binding site, which showed one of the highest binding constant. Taken together, these results raise the possibility of using this luciferase\u2010like enzyme for the development of novel whole\u2010cell luminescent biosensors for diclofenac and similar drugs.<\/jats:p>","DOI":"10.1002\/bio.3952","type":"journal-article","created":{"date-parts":[[2020,9,22]],"date-time":"2020-09-22T07:46:36Z","timestamp":1600760796000},"page":"367-376","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Selective inhibition of <i>Zophobas morio<\/i> (Coleoptera: Tenebrionidae) luciferase\u2010like enzyme luminescence by diclofenac and potential suitability for <i>light\u2010off<\/i> biosensing"],"prefix":"10.1002","volume":"36","author":[{"given":"Mariele C.","family":"Carvalho","sequence":"first","affiliation":[{"name":"Graduate Program of Evolutive Genetics and Molecular Biology Federal University of S\u00e3o Carlos (UFSCar)  S\u00e3o Carlos Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9638-7454","authenticated-orcid":false,"given":"At\u00edlio","family":"Tomazini","sequence":"additional","affiliation":[{"name":"Brazilian Biorenewables National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas  SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rogilene A.","family":"Prado","sequence":"additional","affiliation":[{"name":"Graduate Program of Biotechnology and Environmental Monitoring Federal University of S\u00e3o Carlos (UFSCar)  Sorocaba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5558-9199","authenticated-orcid":false,"given":"Vadim R.","family":"Viviani","sequence":"additional","affiliation":[{"name":"Graduate Program of Evolutive Genetics and Molecular Biology Federal University of S\u00e3o Carlos (UFSCar)  S\u00e3o Carlos Brazil"},{"name":"Graduate Program of Biotechnology and Environmental Monitoring Federal University of S\u00e3o Carlos (UFSCar)  Sorocaba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,11,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/AN9911600753"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2020.114993"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2020.104836"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.138312"},{"key":"e_1_2_7_6_1","first-page":"80","volume":"29","author":"Cuthbert R.","year":"2009","journal-title":"Vet. 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