{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T14:00:59Z","timestamp":1770991259923,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,4,14]],"date-time":"2021-04-14T00:00:00Z","timestamp":1618358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\u03b1\u2010Difluoromethylornithine is an effective medication for the treatment of African Trypanosomiasis and widely distributed for the treatment of hirsutism. This work provides an adequate analytical protocol for the spectrophotometric and the spectrofluorimetric determination of \u03b1\u2010difluoromethylornithine through its interaction with 4\u2010chloro\u20107\u2010nitrobenzofurazan (NBD\u2010chloride) reagent. After optimization of the reaction conditions (NBD\u2010chloride volume, buffer volume, the best diluting solvent, heating time and temperature and pH of the medium) the reaction product was measured spectrophotometrically at <jats:italic>\u03bb<\/jats:italic><jats:sub>max<\/jats:sub> = 478\u2009nm and spectrofluorimetrically at <jats:italic>\u03bb<\/jats:italic><jats:sub>emission<\/jats:sub> = 540\u2009nm after <jats:italic>\u03bb<\/jats:italic><jats:sub>excitation<\/jats:sub> = 475\u2009nm. The proposed methods were linear over the ranges 5\u201330\u2009\u03bcg\u2009ml<jats:sup>\u22121<\/jats:sup> and 0.4\u20132\u00a0\u03bcg\u2009ml<jats:sup>\u22121<\/jats:sup> for the spectrophotometric method and the spectrofluorimetric method, respectively. Moreover, the proposed work offers an adequate sensitive and selective determination for \u03b1\u2010difluoromethylornithine where the detection limits were 0.90\u2009\u03bcg\u2009ml<jats:sup>\u22121<\/jats:sup> and 0.071\u2009\u03bcg\u2009ml<jats:sup>\u22121<\/jats:sup> for the spectrophotometric method and the spectrofluorimetric method, respectively. Furthermore, both methods were successfully applied for the quantification of \u03b1\u2010difluoromethylornithine in the pharmaceutical cream samples with acceptable recovery results.<\/jats:p>","DOI":"10.1002\/bio.4048","type":"journal-article","created":{"date-parts":[[2021,4,5]],"date-time":"2021-04-05T09:10:57Z","timestamp":1617613857000},"page":"1231-1238","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Utility of 4\u2010chloro\u20107\u2010nitrobenzofurazan for spectrofluorimetric and spectrophotometric determinations of the anti\u2010hirsutism agent (\u03b1\u2010difluoromethylornithine) in pharmaceutical cream samples"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9207-7448","authenticated-orcid":false,"given":"Albandary","family":"Almahri","sequence":"first","affiliation":[{"name":"General Courses Unit, Faculty of Sciences and Arts King Khalid University  Dhahran Aljanoub Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,4,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.2165\/00128071-200001020-00003"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3949\/ccjm.77a.08079"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3109\/09513591003686353"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.39485.592674.BE"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1086\/432576"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.2165\/00128071-200102030-00009"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4632.2007.03270.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1185\/0300799019117002"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4632.2006.03079.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(02)00438-5"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2006.08.030"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1361"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600780208"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.3374"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.20902\/IJCTR.2018.111139"},{"key":"e_1_2_7_17_1","first-page":"90","volume":"1","author":"Kumar A.","year":"2013","journal-title":"Amer. 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