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These were subjected to ADME (absorption, distribution, metabolism and excretion), physiochemical properties, molecular docking, <jats:italic>in vitro<\/jats:italic> antibacterial and antioxidant studies. The in silico studies indicated lower toxicity with metabolic stability for nearly all the derivatives proving reliability as drug candidates. The comparative antibacterial study against <jats:styled-content style=\"fixed-case\"><jats:italic>Staphylococcus aureus<\/jats:italic><\/jats:styled-content> and <jats:styled-content style=\"fixed-case\"><jats:italic>Escherichia coli<\/jats:italic><\/jats:styled-content>, also showed a superior inhibition than reference drug and their mono counterparts. The increase in linker alkyl chain length and variation of substituents in indole, further predicted increased inhibition, with maximum value for compound <jats:bold>4o<\/jats:bold> at 50\u2009\u03bcg\/ml. The <jats:italic>in vitro<\/jats:italic> calf thymus DNA (ct\u2010DNA) binding ability of compounds <jats:bold>4c, 4f, 4i, 4l, 4\u00a0m, 4n,<\/jats:bold> and <jats:bold>4o<\/jats:bold> was evaluated via ultraviolet\u2010visible and fluorescence spectroscopy techniques. A hyperchromic effect was observed with no apparent wavelength shift which predicted for the groove binding mode. A moderate binding constant for <jats:bold>4o<\/jats:bold>, in fluorescence results, confirms groove binding. The molecular docking of <jats:bold>4o<\/jats:bold> with ct\u2010DNA (PDBID:1BNA) and SARS\u2010CoV\u20102 M<jats:sup>pro<\/jats:sup> (3CL protease, PDBID:6LU7) prove its efficacy as potential DNA binder and antiviral agent.<\/jats:p>","DOI":"10.1002\/bio.4098","type":"journal-article","created":{"date-parts":[[2021,6,4]],"date-time":"2021-06-04T12:27:12Z","timestamp":1622809632000},"page":"1531-1543","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Synthesis, comparative <i>in vitro<\/i> antibacterial, antioxidant and UV fluorescence studies of bis indole Schiff bases and molecular docking with ct\u2010DNA and SARS\u2010CoV\u20102 M<sup>pro<\/sup>"],"prefix":"10.1002","volume":"36","author":[{"given":"Sugandha","family":"Singhal","sequence":"first","affiliation":[{"name":"University School of Basic and Applied Sciences, Guru Gobind Singh Indraprastha University  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pankaj","family":"Khanna","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Acharya Narendra Dev College University of Delhi  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7438-8245","authenticated-orcid":false,"given":"Leena","family":"Khanna","sequence":"additional","affiliation":[{"name":"University School of Basic and Applied Sciences, Guru Gobind Singh Indraprastha University  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,6,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.7b00108"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.4103\/jgid.jgid_110_18"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules18066620"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2017.04.003"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389450121666200310115327"},{"key":"e_1_2_8_7_1","first-page":"9139786","volume":"2018","author":"Ibrahim M. 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