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The title substrate capable of chemiluminescence (CL) was isolated in a highly pure state as a chloride salt (99.9% using RP\u2010HPLC) and identified using mass spectrometry (ESI Q\u2010TOF) and <jats:sup>1<\/jats:sup>H NMR spectroscopy. The cation included in the salt, 9\u2010CMA<jats:sup>+<\/jats:sup>, underwent oxidation in an alkaline environment containing RSHs by molecular oxygen, generating CL of various intensities, with no need for the use of hydrogen peroxide. The amount of produced light was linearly proportional to the content of investigated analytes in the system over the concentration range ~0.2\u20132\u2009\u03bcM, with the detection limits in the range 0.19\u20131.73\u2009\u03bcM. The mechanism of chemiluminogenic oxidation of 9\u2010CMA<jats:sup>+<\/jats:sup> in the presence of RSHs and molecular oxygen is proposed, using computational methods at the density\u2010functional theory level. The presence of RSHs in an alkaline medium seems to be crucial to produce hydroperoxide anions (<jats:sup>\u2212<\/jats:sup>OOH), which initiate the \u2018light path\u2019 of 9\u2010CMA<jats:sup>+<\/jats:sup> transformations, ending with the excretion of electronically excited molecules of 10 methyl\u2010acridan\u20109\u2010one.<\/jats:p>","DOI":"10.1002\/bio.4162","type":"journal-article","created":{"date-parts":[[2021,11,11]],"date-time":"2021-11-11T21:55:33Z","timestamp":1636667733000},"page":"208-219","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["New luminometric method for quantification of biological sulfur nucleophiles with the participation of 9\u2010cyano\u201010\u2010methylacridinium salt"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2809-7524","authenticated-orcid":false,"given":"Vladyslav","family":"Ievtukhov","sequence":"first","affiliation":[{"name":"Faculty of Chemistry University of Gda\u0144sk  Wita Stwosza Str. 63 Gda\u0144sk Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7125-5233","authenticated-orcid":false,"given":"Beata","family":"Zadykowicz","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry University of Gda\u0144sk  Wita Stwosza Str. 63 Gda\u0144sk Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8032-347X","authenticated-orcid":false,"given":"Mykola Ye.","family":"Blazheyevskiy","sequence":"additional","affiliation":[{"name":"National University of Pharmacy  Valentynivska Str.4 Kharkiv Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4811-961X","authenticated-orcid":false,"given":"Karol","family":"Krzymi\u0144ski","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry University of Gda\u0144sk  Wita Stwosza Str. 63 Gda\u0144sk Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-011-3587-6"},{"key":"e_1_2_7_3_1","first-page":"350","volume":"1","author":"Mashkovsky M. 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