{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T04:46:34Z","timestamp":1776228394408,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A new sensitive and instantaneous spectrofluorimetric method for efficient determination of lomefloxacin (LMX) in its pure, dosage form and human plasma was designed. The developed method depends on formation of a metal\u2010chelation compound of LMX as a ligand with zinc(II) in a buffer of acetate (pH\u00a05.5). The following parameters; type of metal, concentration of metal, pH, type of buffer and diluting solvent were optimized. After carefully investigation; 0.2\u00a0mM zinc, 2.0\u00a0ml acetate buffer (pH 5.5) and water as diluting solvent were set as optimum reaction conditions. Under these conditions, a large increase in the intensity of the fluorescence of LMX was attained at 450 after excitation at 284\u2009nm. The limits of detection and quantification were 5.8 and 1.9 ng\u2009ml<jats:sup>\u22121<\/jats:sup>, respectively, with linearity range of 10.0 to 500.0\u00a0ng\u2009ml<jats:sup>\u22121<\/jats:sup>. The binding mode of LMX and zinc(II) ion (Zn<jats:sup>2+<\/jats:sup>) was found to be 2:1, respectively, and confirmed by Job's plot method. Furthermore, it extended to the analysis of LMX in the spiked plasma of humans with percentage recovery (98.70\u2009\u00b1\u00a00.97 to 100.30\u2009\u00b1\u00a01.69%, <jats:italic>n<\/jats:italic>\u00a0=\u00a03).<\/jats:p>","DOI":"10.1002\/bio.4168","type":"journal-article","created":{"date-parts":[[2021,11,21]],"date-time":"2021-11-21T21:13:53Z","timestamp":1637529233000},"page":"255-262","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A novel spectrofluorimetric method for determination of lomefloxacin adopting on zinc(II) chelation strategy: Application in human plasma"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7241-2130","authenticated-orcid":false,"given":"Tamer Z.","family":"Attia","sequence":"first","affiliation":[{"name":"Analytical Chemistry Department, Faculty of Pharmacy Minia University  Minia Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6635-7606","authenticated-orcid":false,"given":"Mahmoud A.","family":"Omar","sequence":"additional","affiliation":[{"name":"Analytical Chemistry Department, Faculty of Pharmacy Minia University  Minia Egypt"},{"name":"Department of Pharmacognosy and Pharmaceutical Chemistry, College of Pharmacy Taibah University  Medinah Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deena A. 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