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The use of free MAs as antigens for the serodiagnosis of TB is known but has not been developed into a point of care test. This study focuses on the synthesis, solubility, and lateral flow of QDs coupled to MAs. Water\u2010soluble CdSe\/ZnS QDs capped with <jats:sc>l<\/jats:sc>\u2010cysteine were synthesised and covalently coupled to MAs via amide linkages to form a water\u2010soluble fluorescent probe: MA\u2010CdSe\/ZnS QDs. The MA\u2010CdSe\/ZnS QDs showed broad absorption bands and coupling, confirmed by the presence of amide bonds in the Fourier\u2010transform infrared (FTIR) spectrum, resulting in a blue shift in fluorescence. Powder X\u2010ray diffraction (XRD) revealed a shift and increase in the number of peaks for MA\u2010CdSe\/ZnS QDs relative to the L\u2010cys\u2010CdSe\/ZnS QDs, suggesting that coupling changed the crystal structure. The average particle size of MA\u2010CdSe\/ZnS QDs was ~3.0\u00a0nm. Visual paper\u2010based lateral flow of MA\u2010CdSe\/ZnS QDs was achieved on strips of nitrocellulose membrane with both water and membrane blocking solution eluents. The highly fluorescent MA\u2010CdSe\/ZnS QDs showed good water solubility and lateral flow, which are important properties for fluorescence sensing applications.<\/jats:p>","DOI":"10.1002\/bio.4170","type":"journal-article","created":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T09:59:06Z","timestamp":1637661546000},"page":"278-289","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Synthesis and characterisation of quantum dots coupled to mycolic acids as a water\u2010soluble fluorescent probe for potential lateral flow detection of antibodies and diagnosis of tuberculosis"],"prefix":"10.1002","volume":"37","author":[{"given":"Kapambwe P.","family":"Kabwe","sequence":"first","affiliation":[{"name":"Department of Chemistry, Faculty of Natural and Agricultural Sciences University of Pretoria  Pretoria South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sifiso A.","family":"Nsibande","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Natural and Agricultural Sciences University of Pretoria  Pretoria South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yolandy","family":"Lemmer","sequence":"additional","affiliation":[{"name":"CSIR\u2010Next Generation Health  Pretoria South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lynne A.","family":"Pilcher","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Natural and Agricultural Sciences University of Pretoria  Pretoria South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3453-9162","authenticated-orcid":false,"given":"Patricia B. 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