{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T15:12:08Z","timestamp":1774365128158,"version":"3.50.1"},"reference-count":18,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,3,19]],"date-time":"2022-03-19T00:00:00Z","timestamp":1647648000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ertapenem (EPM) has been recently approved by the United States Food and Drug Administration (US\u2010FDA) as an antimicrobial drug. EPM has a broad spectrum of action against different bacterial strains and is most commonly prescribed in Egypt for the treatment of <jats:italic>Klebsiella<\/jats:italic> pneumonia. In this study, EPM was estimated using a sensitive and selective spectrofluorimetric method for human plasma and pharmaceutical vials. The measured fluorescence (at 540\u2009nm) was obtained from reaction of EPM with 0.05% w\/v benzofurazan (NBD\u2010Cl) using 0.1\u2009M borate buffer pH\u20098.8 after excitation at 460\u2009nm. The fluorometric linear range was stable from 10 to 350\u2009ng\u2009ml<jats:sup>\u22121<\/jats:sup>. The lower limit of detection and the lower limit of quantitation were found to be 2.13 and 6.47\u2009ng\u2009ml<jats:sup>\u22121<\/jats:sup> respectively. Many factors such as pH, temperature, heating time, and NBD\u2010Cl concentration were optimized. The presented work was validated according to International Council for Harmonisation guidelines and bio\u2010analytically validated using FDA recommendations. The significant finding of this study, sensitivity, was successfully applied in Egypt for a pharmacokinetic application and commercial vials. Pharmacokinetic parameters were studied and the result, recorded as C<jats:sub>max<\/jats:sub> of EPM, was found to be 83.60\u2009\u03bcg ml<jats:sup>\u22121<\/jats:sup> after infusion of 0.5\u2009g of Invanz\u00ae for 30\u2009min. AUC<jats:sub>0\u2010\u221e<\/jats:sub> was found to be 320\u2009\u00b1\u200930.2\u2009\u03bc.h\u2009ml<jats:sup>\u22121<\/jats:sup>.<\/jats:p>","DOI":"10.1002\/bio.4223","type":"journal-article","created":{"date-parts":[[2022,3,11]],"date-time":"2022-03-11T02:56:46Z","timestamp":1646967406000},"page":"796-802","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Bio\u2010analytically fluorimetric method for estimation of ertapenem in real human plasma and commercial samples; application to pharmacokinetics study"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6410-3725","authenticated-orcid":false,"given":"Baher I.","family":"Salman","sequence":"first","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department Faculty of Pharmacy, Al\u2010Azhar University, Assiut branch  Assiut Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roshdy E.","family":"Saraya","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Port Said University  Port Said Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,3,19]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2018.12.018"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02143-08"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/bio.3688"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/bio.3539"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/bio.3590"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0RA07960J"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2021.120077"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0RA10466C"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11696-019-00824-8"},{"key":"e_1_2_5_11_1","doi-asserted-by":"crossref","first-page":"184","DOI":"10.25004\/IJPSDR.2013.050410","volume":"5","author":"Babu K. 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