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It has ravaged several countries and burdened many healthcare systems. As the process of authorizing a novel treatment for human use is extensive and involves multiple phases to obtain safety information and identify potential concerns. Therefore, the fastest and easiest choice was to use United States Food and Drug Administration (US FDA)\u2010approved drugs such as favipiravir and hydroxychloroquine. For the simultaneous estimation of both medications, a simple synchronous spectrofluorimetric approach was established in which both drugs were measured at 372 and 323\u2009nm, respectively in the presence of each other without interference at \u0394\u03bb 60\u2009nm. The effect of various experimental conditions on synchronous fluorescence intensities were thoroughly investigated and optimized. The maximum synchronous fluorescence intensities were obtained at pH\u00a05.4 using acetate buffer (0.2\u00a0M, 0.5\u00a0ml) and ethanol as a diluent. Excellent linearity ranges were obtained using 1.0\u201318.0\u00a0ng\/ml and 10.0\u2013120.0\u00a0ng\/ml for favipiravir and hydroxychloroquine, respectively. The approach exhibited high sensitivity with detection limits down to 0.25\u2009ng\/ml and 1.52\u2009ng\/ml and quantitation limits down to 0.77\u2009ng\/ml and 4.62\u2009ng\/ml, respectively. Spiking human plasma samples with the studied drugs yielded high % recoveries, allowing a significant bioanalytical application. Moreover, the method was validated according to International Conference on Harmonization guidelines and further applied to commercial pharmaceutical preparations with good results.<\/jats:p>","DOI":"10.1002\/bio.4240","type":"journal-article","created":{"date-parts":[[2022,3,28]],"date-time":"2022-03-28T06:13:56Z","timestamp":1648448036000},"page":"953-964","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Quantitative analysis of favipiravir and hydroxychloroquine as FDA\u2010approved drugs for treatment of COVID\u201019 using synchronous spectrofluorimetry: application to pharmaceutical formulations and biological fluids"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4922-9442","authenticated-orcid":false,"given":"Mona E.","family":"El Sharkasy","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7968-6487","authenticated-orcid":false,"given":"Manar M.","family":"Tolba","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fathalla","family":"Belal","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed","family":"Walash","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4013-3110","authenticated-orcid":false,"given":"Rasha","family":"Aboshabana","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,4,10]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"1","volume":"5","author":"Cascella M.","year":"2021","journal-title":"StatPearls"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eng.2020.03.007"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2021.106189"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.111518"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-90551-6"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1811345115"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40262-015-0364-1"},{"key":"e_1_2_9_9_1","first-page":"1","volume":"5","author":"Ivashchenko A. 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