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The U<jats:sup>VI<\/jats:sup> reagent offers a selective fluorescence enhancement behaviour at emission wavelength\u2009=\u2009557\u2009nm. The parameters affecting fluorometric detection of uranium ions, such as the pH, solvent type, ligand concentration, interaction time, and interfering ions, were investigated and adjusted. The proposed U<jats:sup>VI<\/jats:sup> reagent can detect and determine uranium ions even at low concentrations, for which the obtained limit of detection was 0.1\u2009ppm. Additionally, this proposed determination protocol was successfully used to detect, monitor, and determine uranium ions in actual water samples.<\/jats:p>","DOI":"10.1002\/bio.4253","type":"journal-article","created":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T05:05:21Z","timestamp":1649739921000},"page":"1001-1008","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["A novel spectrofluorimetric method based on a reaction with an azoisoxazoles\u2013benzenesulfonamide derivative for determination of uranium (VI) ions in water samples"],"prefix":"10.1002","volume":"37","author":[{"given":"Zaki","family":"Badr","sequence":"first","affiliation":[{"name":"Chemistry Department, Faculty of Science Al\u2010Azhar University  Assiut Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hassanien","family":"Gomaa","sequence":"additional","affiliation":[{"name":"Chemistry Department, Faculty of Science Al\u2010Azhar University  Assiut Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed S. 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