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Catalase is a key antioxidant enzyme that is closely relevant to the level of reactive oxygen specie <jats:italic>in vivo<\/jats:italic>. Here, the research explored the effects of NDP on the conformation and catalytic function of bovine liver catalase (BLC) through enzymatic reaction kinetic techniques, multispectroscopic analysis, and computer simulation methods. Kinetic studies clarified that the NDP reduced the activity of BLC using a noncompetitive inhibition mechanism. Based on trial data, a static quenching mechanism functioned in quenching the intrinsic fluorescence of BLC. The binding constant value was (4.486\u2009\u00b1\u20090.008)\u2009\u00d7\u200910<jats:sup>4<\/jats:sup>\u2009M<jats:sup>\u22121<\/jats:sup> (298\u2009K) and BLC had one binding site for NDP. Tyr was prone to be exposed more to a hydrophilic environment in wake of a shift in fluorescence value. The binding reaction of BLC to NDP caused a conformational change in BLC, which in turn led to increase in the \u03b1\u2010helix content and a decline in the \u03b2\u2010sheet content. Furthermore, several amino acids residues interacted with NDP by means of van der Waals forces, whereas Gln397, Asn368, Gln371, Asn384, and Pro377 formed several hydrogen bonds with NDP.<\/jats:p>","DOI":"10.1002\/bio.4330","type":"journal-article","created":{"date-parts":[[2022,7,11]],"date-time":"2022-07-11T08:59:45Z","timestamp":1657529985000},"page":"1547-1556","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Elucidation on inhibition and binding mechanism of bovine liver catalase by nifedipine: multispectroscopic analysis and computer simulation methods"],"prefix":"10.1002","volume":"37","author":[{"given":"Like","family":"Zhou","sequence":"first","affiliation":[{"name":"School of Chemistry and Chemical Engineering Nanchang University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Chemistry 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