{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T07:57:34Z","timestamp":1773907054425,"version":"3.50.1"},"reference-count":11,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,1,21]],"date-time":"2003-01-21T00:00:00Z","timestamp":1043107200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2003,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Allele\u2010specific PCR for <jats:italic>E. coli<\/jats:italic> O157 was conducted with primers specific to verotoxin genes, verotoxin 1 (VT1) and verotoxin 2 (VT2). VT is an important cause of haemorrhagic colitis (HC) and haemolytic uraemic syndrome (HUS) worldwide. We developed a simple, rapid bioluminescent detection method for <jats:italic>E. coli<\/jats:italic> O157. The method is based on the determination of pyrophosphoric acid (PPi) released during allele\u2010specific PCR. Thus, released PPi is converted to ATP by ATP sulphurylase and the concentration of ATP is determined using the firefly luciferase reaction. As a result, VT1, VT2 and DNA with VT1\/VT2 were clearly identified by this method. This protocol, which does not require expensive equipment, can be utilized to monitor the PCR product rapidly. Additionally, this methodology can be used as a high\u2010throughput approach for measuring PCR products. Copyright \u00a9 2003 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bio.437","type":"journal-article","created":{"date-parts":[[2003,4,7]],"date-time":"2003-04-07T11:03:40Z","timestamp":1049713420000},"page":"107-112","source":"Crossref","is-referenced-by-count":11,"title":["Simple and rapid bioluminescent detection of two verotoxin genes using allele\u2010specific PCR of <i>E. coli<\/i> O157: H7"],"prefix":"10.1002","volume":"18","author":[{"given":"O.","family":"Imamura","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Arakawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Maeda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,1,21]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.1993.tb01681.x"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1128\/jcm.33.3.519-524.1995","article-title":"Direct detection of verotoxin\u2010producing Escherichia coli in stool sample by PCR","volume":"33","author":"Ramotar K","year":"1995","journal-title":"J. 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