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These approaches depend on the reaction of the tertiary amine group of nilotinib with erythrosine B in the Britton\u2013Robinson buffer at pH\u00a04. Method I, depends on measuring the absorbance of the formed complex at 551\u2009nm. The absorbance concentration plot showed linearity over the concentration range of 1.0 to 9.0\u00a0\u03bcg\/ml. Method II, involved the measurement of the quenching of the native fluorescence of erythrosine B by adding nilotinib in an acidic medium. The fluorescence quenching of erythrosine B was measured at 549\u2009nm after excitation at 528\u2009nm. This approach showed excellent linearity in the concentration range of 0.04 to 0.7\u00a0\u03bcg\/ml. The limit of detection values for Method I and Method II were 0.225 and 0.008\u2009\u03bcg\/ml, respectively, while the limit of quantitation values for Method I and Method II were 0.68 and 0.026\u2009\u03bcg\/ml, respectively. To get the optimal conditions, factors that may affect the formation of the ion\u2010pairing complex were thoroughly examined. The two approaches were carefully validated following the International Conference of Harmonization (ICH Q2R1) guidelines. Statistical assessment of the results achieved using the suggested and previously published comparison approaches showed no significant difference. The approaches were successful in determining nilotinib in a pharmaceutical dosage form as well as spiked human plasma samples. The eco\u2010friendly properties of the methods were evaluated by three different tools.<\/jats:p>","DOI":"10.1002\/bio.4448","type":"journal-article","created":{"date-parts":[[2023,1,26]],"date-time":"2023-01-26T19:44:35Z","timestamp":1674762275000},"page":"291-301","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Utility of a xanthene\u2010based dye for determination of nilotinib using two spectroscopic approaches. Applications to bulk powder, capsules, and spiked human plasma"],"prefix":"10.1002","volume":"38","author":[{"given":"Diaa","family":"Dagher","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3953-7169","authenticated-orcid":false,"given":"Heba","family":"Elmansi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenny Jeehan","family":"Nasr","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nahed","family":"El\u2010Enany","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Mansoura University  Mansoura Egypt"},{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy New Mansoura University  New Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,2,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-017-2127-x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-131-3-199908030-00008"},{"key":"e_1_2_7_4_1","first-page":"653","volume":"7","author":"Gale R. 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