{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T14:39:59Z","timestamp":1773671999560,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,5,6]],"date-time":"2023-05-06T00:00:00Z","timestamp":1683331200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Doxepin hydrochloride (DOX) is a tricyclic antidepressant drug. Three sensitive spectrofluorimetric methods, namely resonance Rayleigh scattering (RRS), frequency doubling scattering (FDS) and second\u2010order scattering (SOS), were developed and validated for their estimation of doxepin in spiked human plasma and formulation using liquid\u2013liquid extraction method through the formation of an ion pair complex with eosin Y at a pH of\u00a04.5. Various factors affecting fluorescence intensity were optimized, and the reaction kinetics was determined using the Arrhenius equation method. Different scattering methods such as RRS, FDS and SOS were developed at maximum scattering wavelengths <jats:italic>\u03bb<\/jats:italic><jats:sub>ex<\/jats:sub>\/<jats:italic>\u03bb<\/jats:italic><jats:sub>em<\/jats:sub>\u2009=\u2009567\/567\u2009nm for RRS, 720\/360\u2009nm for SOS and 260\/520\u2009nm for FDS, respectively. The methods exhibited high sensitivities, and the detection limits for DOX were found to be 0.82, 1.20 and 1.03\u2009ng\/ml for RRS, FDS and SOS methods, respectively. The FDS method exhibited the highest sensitivity. The methods were validated using the International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use guidelines and applied to determine DOX in capsule and spiked human plasma samples.<\/jats:p>","DOI":"10.1002\/bio.4503","type":"journal-article","created":{"date-parts":[[2023,4,15]],"date-time":"2023-04-15T00:03:55Z","timestamp":1681517035000},"page":"753-761","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Determination of doxepin hydrochloride in spiked human plasma and capsule after derivatization with eosin Y using resonance Rayleigh scattering, second\u2010order scattering and frequency doubling scattering methods"],"prefix":"10.1002","volume":"38","author":[{"given":"Jeeva","family":"Geogin","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Chemistry and Analysis, K.B. Institute of Pharmaceutical Education and Research Kadi Sarva Vishwavidyalaya  Gandhinagar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madhuri","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry and Analysis, K.B. Institute of Pharmaceutical Education and Research Kadi Sarva Vishwavidyalaya  Gandhinagar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nishith","family":"Teraiya","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry and Analysis, K.B. Institute of Pharmaceutical Education and Research Kadi Sarva Vishwavidyalaya  Gandhinagar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashlesha","family":"Chauhan","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry and Analysis, K.B. 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