{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T18:20:39Z","timestamp":1778091639763,"version":"3.51.4"},"reference-count":52,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2023,7,13]],"date-time":"2023-07-13T00:00:00Z","timestamp":1689206400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100005156","name":"Alexander von Humboldt-Stiftung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005156","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Moxifloxacin and ofloxacin are two broad\u2010spectrum quinolone antibiotics. They are among the most widely used antibiotics, at this time, applied to control the COVID\u201019 pandemic. Hydroxychloroquine is an FDA\u2010approved drug for the treatment of COVID\u201019. This work describes a simple, green, selective, and sensitive spectrofluorimetric method for the assay of moxifloxacin and ofloxacin in the presence of hydroxychloroquine, two co\u2010administered mixtures used in the treatment of hospital\u2010acquired pneumonia in patients with COVID\u201019. Simultaneous assay of hydroxychloroquine and moxifloxacin was carried out in methanol using a direct spectrofluorimetric method (method I) at 375 and 550\u2009nm, respectively, after excitation at 300\u2009nm. The direct spectrofluorimetric assay was rectilinear over concentration ranges 50.0\u2013400.0 and 300.0\u20132500.0 ng\/ml for hydroxychloroquine and moxifloxacin, respectively, with limits of detection (LOD) of 6.4 and 33.64\u2009ng\/ml and limits of quantitation (LOQ) of 19.4 and 102.6\u00a0ng\/ml, respectively, for the two drugs. The assay for hydroxychloroquine and ofloxacin was carried out by measuring the first derivative synchronous amplitude for hydroxychloroquine at the zero crossing point of ofloxacin and vice versa at \u0394\u03bb\u2009=\u2009140\u2009nm (method II). Hydroxychloroquine was measured at 266\u2009nm, while ofloxacin was measured at 340\u2009nm over the concentration range 4\u201340\u2009ng\/ml for hydroxychloroquine and 200\u20132000\u2009ng\/ml for ofloxacin with LOD of 0.467 and 25.3\u00a0ng\/ml and LOQ of 1.42 and 76.6\u00a0ng\/ml, respectively, for the two drugs. The two methods were validated following International Conference on Harmonization guidelines and were applied to the analysis of the two drugs in plasma with good percentage recoveries (109.73\u201393.17%).<\/jats:p>","DOI":"10.1002\/bio.4539","type":"journal-article","created":{"date-parts":[[2023,6,19]],"date-time":"2023-06-19T19:53:15Z","timestamp":1687204395000},"page":"1572-1582","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Validated spectrofluorimetric assay of two co\u2010administered drug mixtures containing hydroxychloroquine with either moxifloxacin or ofloxacin as a drug regimen for hospital\u2010acquired pneumonia in patients with COVID\u201019"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8019-2924","authenticated-orcid":false,"given":"Asmaa","family":"El\u2010Sayed","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3953-7169","authenticated-orcid":false,"given":"Heba mohamed","family":"Elmansi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1468-1367","authenticated-orcid":false,"given":"Shereen","family":"Shalan","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manal","family":"Eid","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,7,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2020.1775132"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-020-03013-2"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2020.07.016"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiph.2021.02.001"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-198836020-00004"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0149-2918(00)88266-1"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jemermed.2007.08.068"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.2174\/1568005043340920"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.3851\/IMP1937"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s43440-020-00169-0"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-200464200-00006"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ardp.201800141"},{"key":"e_1_2_7_14_1","first-page":"46","volume":"46","author":"Connor E.","year":"2022","journal-title":"Commun. 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