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The plant biomolecules induce the reduction of Cu<jats:sup>2+<\/jats:sup> ions to CuNPs and also act as a capping and stabilizing agent. The formation of CuNPs was confirmed using photoluminescence (PL) excitation and emission spectra on a Shimadzu RF\u20105301 PC spectrofluorophotometer and the absorbance spectra of a UV\u2013visible spectrophotometer at different stages during the synthesis process. In addition, other properties of synthesized CuNPs were also investigated using X\u2010ray diffraction, Fourier transform infrared spectroscopy, and transmission electron microscopy techniques. The average size of the synthesized CuNPs was in the range 20\u201340\u2009nm. Furthermore, the synthesized NPs were also considered for an antimicrobial study against Gram\u2010positive <jats:styled-content style=\"fixed-case\"><jats:italic>Staphylococcus aureus<\/jats:italic><\/jats:styled-content> and <jats:italic>Proteus<\/jats:italic>, and Gram\u2010negative <jats:styled-content style=\"fixed-case\"><jats:italic>Escherichia coli<\/jats:italic><\/jats:styled-content> and <jats:italic>Salmonella<\/jats:italic> spp. using the agar well diffusion method. The zone of inhibition against the Gram\u2010positive bacteria was greater than the zone of inhibition against the Gram\u2010negative bacteria. These investigation results suggest that synthesized NPs are promising nanomaterials for use as antimicrobial agents.<\/jats:p>","DOI":"10.1002\/bio.4579","type":"journal-article","created":{"date-parts":[[2023,8,11]],"date-time":"2023-08-11T03:26:54Z","timestamp":1691724414000},"page":"1912-1920","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Green synthesis\u2010assisted copper nanoparticles using <scp><i>Aegle marmelos<\/i><\/scp> leaves extract: physical, optical, and antimicrobial properties"],"prefix":"10.1002","volume":"38","author":[{"given":"Shruti","family":"Tanwar","sequence":"first","affiliation":[{"name":"Department of Microbiology Taywade College  Mahadula\u2010Koradi Nagpur India"},{"name":"Department of Physics, R.T.M. 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