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Due to their massive, combined administration in many illnesses, there is a persistent need for their concurrent estimation in different pharmaceutical formulations. Two sensitive, environmentally friendly spectrofluorimetric methods were developed. AMX was determined using the first method (I) without interference from GFN. This method depends on the quenching of Erythrosine B (EB) native fluorescence at 552\u2009nm after excitation at 527\u2009nm due to the formation of a non\u2010fluorescent AMX\u2010EB ion\u2010pair complex in Britton\u2013Robinson buffer (BRB) solution pH (3.5). The concentration plot is linear over the 0.25\u20135.0\u00a0\u03bcg\/mL range, with a mean percent found value of 99.74%. Method (II) depends on measuring the native fluorescence of aqueous GFN solution at two analytical wavelengths, either 300 or 600\u2009nm, after excitation at 274\u2009nm. Relative fluorescence intensity (RFI)\u2013concentration plots are linear over the ranges of 0.02\u20130.5 and 0.1\u20132.0\u00a0\u03bcg\/ml, with mean percent found at 99.96% and 99.91% at dual wavelengths, respectively. The proposed methods were successfully applied to assay both drugs in raw materials and different single and combined pharmaceutical formulations. These methods have been thoroughly validated following International Committee on Harmonisation (ICH) guidelines. National Environmental Methods Index, Analytical Eco\u2010Scale, and Green Analytical Procedure Index were used to prove greenness, thereby enhancing their applicability. The proposed techniques provide straightforward, precise, and cost\u2010effective solutions for routine formulation analysis in quality control laboratories.<\/jats:p>","DOI":"10.1002\/bio.4711","type":"journal-article","created":{"date-parts":[[2024,3,19]],"date-time":"2024-03-19T06:00:22Z","timestamp":1710828022000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Two green and sensitive spectrofluorimetric approaches for determination of Ambroxol and guaifenesin in their single and combined pharmaceutical formulations"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-7245-8466","authenticated-orcid":false,"given":"Ghidaa G.","family":"Elawadi","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7122-3760","authenticated-orcid":false,"given":"Fawzi","family":"Elsebaei","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mona E.","family":"Fathy","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed E.\u2010S.","family":"Metwally","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,3,19]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"Martindale: The Complete Drug Reference","author":"Buckingham R.","year":"2020"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/gels7040243"},{"key":"e_1_2_9_4_1","first-page":"281","volume":"73","author":"Wang X.","year":"2015","journal-title":"Biochem. 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