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The viscosity directly affects the metabolism of lipid droplets, and the abnormal viscosity is associated with the occurrence and development of various diseases. Therefore, it is indispensable to develop techniques that can detect viscosity changes in intracellular lipid droplets. Based on twisted intramolecular charge transfer (TICT) mechanism, a novel small\u2010molecule lipid droplet\u2010targeted viscosity fluorescence probe PPF\u20101 was designed. The probe was easy to synthesize, it had a large Stokes shift, stable optical properties, and low bio\u2010toxicity. Compared to being in methanol solution, the fluorescence intensity of PPF\u20101 in glycerol solution was increased 26.7\u2010fold, and PPF\u20101 showed excellent ability to target lipid droplets. Thus, the probe PPF\u20101 could provide an effective means of detecting viscosity changes of lipid droplets and was of great value for physiological diagnosis of related diseases, pathological analysis, and medical research.<\/jats:p>","DOI":"10.1002\/bio.4749","type":"journal-article","created":{"date-parts":[[2024,4,24]],"date-time":"2024-04-24T23:15:59Z","timestamp":1714000559000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["An efficient lipid droplet\u2010targeted fluorescent probe for detection of intracellular viscosity"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-7150-8370","authenticated-orcid":false,"given":"Yingjie","family":"Yang","sequence":"first","affiliation":[{"name":"Institute of Optical Materials and Chemical Biology, Guangxi Key Laboratory of Electrochemical Energy Materials, School of Chemistry and Chemical Engineering Guangxi University  Nanning Guangxi P. 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