{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:51:17Z","timestamp":1759971077368,"version":"build-2065373602"},"reference-count":28,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T00:00:00Z","timestamp":1714608000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002383","name":"King Saud University","doi-asserted-by":"publisher","award":["RSP2024R516"],"award-info":[{"award-number":["RSP2024R516"]}],"id":[{"id":"10.13039\/501100002383","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007470","name":"Shaqra University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007470","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Prucalopride (PCD), is a modern medication approved by the United States in 2018 to alleviate constipation caused by motility issues. PCD demonstrates a strong affinity and selectivity toward the 5\u2010HT4 receptor. The study here introduces a feasible, direct, non\u2010extractive, and affordable pathway for PCD analytical tracking. The fluorimetric study is based on the on\u2013off effect on the emission amplitude of fluorone\u2010based dye (pyrosin B). In a one\u2010pot experiment, the complex between PCD and pyrosin B is formed instantly in an acidic medium. Correlation between decreased pyrosin B emission and PCD concentrations provides a linear calibration plot from 50 to 900\u2009ng\/mL. PCD\u2013dye complex system affecting variables were meticulously tuned. The values of the estimated limit of quantitation and limit of detection for the current methodology were 47.5 and 15.7\u00a0ng\/mL, respectively. Conformity of the strategy validity was achieved by a comprehensive study of the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use criteria. The method was convincingly applied for PCD assay in tablets and content uniformity investigation. Furthermore, PCD tracking in the spiked biological fluid was applied. Finally, the method uses distilled water as dispersing medium which rise accommodation with the green chemistry principle.<\/jats:p>","DOI":"10.1002\/bio.4752","type":"journal-article","created":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T21:04:35Z","timestamp":1714683875000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["An ingenious technique based on the usage of fluorone\u2010based dye; pyrosin B in prucalopride assay in different matrices through an \u201con\u2013off\u201d dye native fluorescence probe"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8524-6867","authenticated-orcid":false,"given":"Ahmed A.","family":"Abu\u2010hassan","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Al\u2010Azhar University Assiut Branch  Assiut Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wael A.","family":"Mahdi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, College of Pharmacy King Saud University  Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sultan","family":"Alshehri","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, College of Pharmacy King Saud University  Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9774-0587","authenticated-orcid":false,"given":"Mohamed A.","family":"El Hamd","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, College of Pharmacy Shaqra University  Shaqra Saudi Arabia"},{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy South Valley University  Qena Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,5,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.2165\/11204000-000000000-00000"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/1756283X11423706"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/1756283X17734809"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.25004\/IJPSDR.2020.120211"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2016.09.006"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/22297928.2021.1911680"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.26463\/rjps.11_3_6"},{"key":"e_1_2_10_9_1","first-page":"15","volume":"66","author":"Eleryan R. 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