{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T23:06:53Z","timestamp":1787612813232,"version":"build-2736575974"},"reference-count":51,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,5,16]],"date-time":"2024-05-16T00:00:00Z","timestamp":1715817600000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100005156","name":"Alexander von Humboldt-Stiftung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005156","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>One of the most common features of many different clinical conditions is pain; hence, there is a crucial need for eliminating or reducing it to a tolerable level to retrieve physical, psychological and social functioning. A first derivative synchronous spectrofluorimetry technique is proposed for the simultaneous determination of celecoxib and tramadol HCl, a recent coformulation authorized for treating acute pain in adults. The method includes using synchronous spectrofluorimetry at \u2206\u03bb\u2009=\u200980\u2009nm where tramadol HCl was determined using first derivative technique at \u03bb\u2009=\u2009230.2\u00a0nm, while celecoxib was determined at \u03bb\u2009=\u2009288.24\u2009nm. The proposed method was successfully applied to their co\u2010formulated dosage forms in addition to spiked human plasma and validated in agreement with the guidelines of the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use (ICH). The linear ranges were found to be 0.50\u20135.0 and 0.15\u20130.50, the limits of detection to be 0.088 and 0.011 and the limits of quantification to be 0.266 and 0.032\u2009\u03bcg\/ml for celecoxib and tramadol, respectively. Statistical analysis revealed no significant difference when compared with previously reported methods as evidenced by the values of the variance ratio F\u2010test and Student <jats:italic>t<\/jats:italic>\u2010test. The proposed method was successfully applied to commercial dosage forms and spiked human samples. Moreover, the greenness of the proposed method was investigated based on the analytical eco\u2010scale approach, with the results showing an excellent green scale with a score of 95.<\/jats:p>","DOI":"10.1002\/bio.4774","type":"journal-article","created":{"date-parts":[[2024,5,17]],"date-time":"2024-05-17T02:29:37Z","timestamp":1715912977000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["First derivative synchronous spectrofluorimetric method for the simultaneous determination of tramadol and celecoxib in their dosage forms and human plasma"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6532-5373","authenticated-orcid":false,"given":"Hesham Sameh","family":"Ramadan","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry Faculty of Pharmacy Suez Canal University  Ismailia Egypt"},{"name":"Department of Pharmaceutical Chemistry Faculty of Pharmacy Horus University\u2010 Egypt  New Damietta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Randa A.","family":"Abdel Salam","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry Faculty of Pharmacy Suez Canal University  Ismailia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghada M.","family":"Hadad","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry Faculty of Pharmacy Suez Canal University  Ismailia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fathalla","family":"Belal","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4429-6504","authenticated-orcid":false,"given":"Mohamed M.","family":"Salim","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Faculty of Pharmacy Horus University\u2010 Egypt  New Damietta Egypt"},{"name":"Department of Pharmaceutical Analytical Chemistry Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,5,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10067-006-0311-5"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1807061"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(99)03313-9"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMp0908913"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2044.2007.05296.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2004.02.005"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1081\/DDC-120005627"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.2147\/JPR.S322292"},{"key":"e_1_2_9_10_1","first-page":"133","volume":"1","author":"Gadge M.","year":"2020","journal-title":"Int. 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