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Viloxazine hydrochloride, a non\u2010stimulant medication, has recently gained approval for treating attention\u2010deficit hyperactivity disorder. This paper describes the first spectrofluorimetric method for precisely measuring the content of viloxazine in pharmaceutical capsules and rat plasma. This method employed NBD\u2010Cl (4\u2010chloro\u20107\u2010nitrobenzo\u20102\u2010oxa\u20101,3\u2010diazole) as a fluorescent probe, which transformed viloxazine in an alkaline environment into a remarkably sensitive fluorescent adduct. Upon excitation at 476\u2009nm, this adduct becomes detectable at a wavelength of 536\u2009nm. The method was validated using ICH criteria, revealing acceptable linearity across a concentration range of 200\u20132000\u2009ng\/ml and high sensitivity with LOD and LOQ values of 46.774\u2009ng\/ml and 141.741\u2009ng\/ml, respectively. This method was adeptly applied in a pharmacokinetic study of viloxazine in rat plasma following a single oral dose (10\u00a0mg\/kg), yielding a mean peak plasma concentration (C<jats:sub>max<\/jats:sub>) of 1721\u2009ng\/ml, achieved within 1.5\u00a0h. Furthermore, the environmental impact of the technique was assessed using two greenness assessment tools, revealing a notable level of eco\u2010friendliness and sustainability.<\/jats:p>","DOI":"10.1002\/bio.4812","type":"journal-article","created":{"date-parts":[[2024,7,5]],"date-time":"2024-07-05T03:48:20Z","timestamp":1720151300000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Development of fluorescence probe for spectrofluorimetric determination of viloxazine hydrochloride in pharmaceutical form and rat plasma; additional pharmacokinetic study"],"prefix":"10.1002","volume":"39","author":[{"given":"Atiah H.","family":"Almalki","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Chemistry College of Pharmacy Taif University  P.O. Box 11099 Taif Saudi Arabia"},{"name":"Addiction and Neuroscience Research Unit, Health Science Campus Taif University  P.O. Box 11099 Taif Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reem M.","family":"Alnemari","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics and Industrial Pharmacy College of Pharmacy Taif University  P.O. Box 11099 Taif Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maram H.","family":"Abduljabbar","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology College of Pharmacy Taif University  Taif Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed H.","family":"Abdelazim","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department Faculty of Pharmacy Al\u2010Azhar University  Nasr City Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3810\/pgm.2010.09.2206"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1176\/ajp.152.12.1793"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2018.10.009"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molstruc.2023.137326"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40263-021-00825-w"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpsyt.2021.789982"},{"key":"e_1_2_9_8_1","first-page":"77","volume":"11","author":"Priya K. 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