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With its frequent usage in pharmacies, there arises a pressing need for a cost\u2010effective, easily implementable, environmentally sustainable detection method with high sensitivity. This study presents a novel signal\u2010on fluorimetric method for detecting OLP in both its pure form and aqueous humor. The proposed approach depends on enhancing the weak intrinsic fluorescence emission of OLP, achieving a remarkable increase of up to 680% compared to its intrinsic fluorescence. This enhancement is achieved by forming micelles around protonated OLP using an acetate buffer (pH\u20093.6) and incorporating a solution of sodium dodecyl sulfate (SDS) surfactant. A strong correlation (<jats:italic>R<\/jats:italic>\u2009=\u20090.9996) is observed between the concentration of OLP and fluorescence intensities ranging from 1.0 to 100.0\u2009ng\u2009mL<jats:sup>\u22121<\/jats:sup> with a limit of detection of 0.22\u2009ng\u2009mL<jats:sup>\u22121<\/jats:sup>. This described method is successfully employed for quantifying OLP in both its powder form and pharmaceutical eye drops. Furthermore, it demonstrates robust performance in determining OLP in artificial aqueous humor with a percentage recovery of 99.05\u2009\u00b1\u20091.51, with minimal interference from matrix interferents. Moreover, the greenness of the described method was evaluated.<\/jats:p>","DOI":"10.1002\/bio.4814","type":"journal-article","created":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T06:29:34Z","timestamp":1721111374000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Green and sensitive detection of olopatadine in aqueous humor using a signal\u2010on fluorimetric approach: GREEnness assessment"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3703-7221","authenticated-orcid":false,"given":"Islam M.","family":"Mostafa","sequence":"first","affiliation":[{"name":"Analytical Chemistry Department, Faculty of Pharmacy Minia University  Minia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5245-8767","authenticated-orcid":false,"given":"Mahmoud A.","family":"Omar","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy and Pharmaceutical Chemistry, College of Pharmacy Taibah University  Al\u2010Madinah Al\u2010Munawwarah Saudi Arabia"},{"name":"Pharmaceutical Analysis Department, Faculty of Pharmacy Minia University  Minia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deena A. 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