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This study describes the tuning \u201cON\u201d the intrinsic fluorescence of FEX by switching \u201cOFF\u201d its intramolecular photoinduced electron transfer (PET) through the protonation of the piperidinyl nitrogen atom using sulfuric acid. The resulting fluorescence was utilized as a basis for the development of a highly sensitive microwell spectrofluorimetric assay (MW\u2010SFA) for the one\u2010step determination of FEX in pharmaceutical tablets and plasma. The linear range of the assay was 10\u2013500\u2009ng\u2009ml<jats:sup>\u22121<\/jats:sup>, and its limit of quantitation was 25.9\u00a0ng\u2009ml<jats:sup>\u22121<\/jats:sup>. The proposed MW\u2010SFA was successfully applied to analyze FEX in pharmaceutical tablets and plasma samples, demonstrating good accuracy and precision. The greenness of the assay was confirmed using three metric assessment tools. In conclusion, the MW\u2010SFA is a straightforward, single\u2010step analysis that requires no experimental adjustments. It offers high sensitivity, efficient sample processing, and environmental sustainability. This assay is highly recommended for pharmaceutical quality control and clinical lab use, particularly for measuring FEX levels.<\/jats:p>","DOI":"10.1002\/bio.4818","type":"journal-article","created":{"date-parts":[[2024,7,14]],"date-time":"2024-07-14T23:09:54Z","timestamp":1720998594000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Tuning fluorescence of fexofenadine by switching OFF intramolecular photoinduced electron transfer: Application to development of one\u2010step green and high throughput microwell spectrofluorimetric assay for analysis of tablets and plasma"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3821-623X","authenticated-orcid":false,"given":"Ibrahim A.","family":"Darwish","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Chemistry, College of Pharmacy King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Majed","family":"Al\u2010Outaibi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, College of Pharmacy King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nourah Z.","family":"Alzoman","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, College of Pharmacy King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,14]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"Fexofenadine Hydrochoride; The Ultimate Step\u2010By\u2010Step Guide","author":"Blokdijk G. 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