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This medication is specifically indicated for preventing blood clots post hip or knee replacement surgeries and in patients with a prior history of clots. Compared to warfarin, dabigatran serves as a viable alternative that does not necessitate routine blood monitoring tests. The complimentary benefits associated with SALL (salting\u2010out assisted liquid\u2013liquid extraction) and the fluorogenic capabilities of benzofurazan. These methods were combined to provide an affordable and sensitive DBG assaying method. The spectral strength of the yellow luminous product was examined at 533.8\u00a0nm and by adjustment of a wavelength of 474.7\u00a0nm for excitation. To assess its linearity, the calibration chart was tested across a DBG concentration range of 30\u2013500\u2009ng\/ml. Via accurate computation based on ICH, the detection limit (LD) was determined to be 9.5\u00a0ng\/ml, and the strategy can quantify the DBG to a limit of 28\u2009ng\/ml. To ensure success, various crucial parameters for method implementation have been extensively studied and adapted. The validation of the strategy adhered to the policies outlined by ICH, affirming its precision in quantifying DBG in capsules. Furthermore, the inclusion of SALLE steps facilitated accurate monitoring of DBG in plasma samples, introducing a unique and advanced methodology for analyzing this compound in biological samples.<\/jats:p>","DOI":"10.1002\/bio.4824","type":"journal-article","created":{"date-parts":[[2024,7,14]],"date-time":"2024-07-14T23:20:05Z","timestamp":1720999205000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Dual benefits of NBD\u2010Cl fluorogenic action and sample pretreatment (SALLE) technology in the assessment of anticoagulant medication: Dabigatran in pharmaceutical capsules and plasma samples"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9774-0587","authenticated-orcid":false,"given":"Mohamed A.","family":"El Hamd","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Chemistry, College of Pharmacy Shaqra University  Shaqra Saudi Arabia"},{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy South Valley University  Qena Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wael A.","family":"Mahdi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, College of Pharmacy King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sultan","family":"Alshehri","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, College of Pharmacy King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8524-6867","authenticated-orcid":false,"given":"Ahmed A.","family":"Abu\u2010hassan","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Al\u2010Azhar University  Assiut Branch Assiut Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13181-011-0178-y"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.110.203604"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinthera.2012.09.008"},{"key":"e_1_2_8_5_1","first-page":"1275","volume":"8","author":"Devi B. 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