{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T01:48:14Z","timestamp":1781660894798,"version":"3.54.5"},"reference-count":56,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,2]],"date-time":"2025-01-02T00:00:00Z","timestamp":1735776000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>A rapid, facile, and green spectrofluorometric method was developed for the concurrent precise estimation of itraconazole and ibuprofen. The developed method involved the use of Tween\u201080 micelle as a green sample matrix for the efficient assay of the analytes of interest. Besides the greenness of Tween\u201080, it significantly enhanced the native fluorescence of itraconazole by about 450%. The spectrofluorometric measurements of studied analytes were conducted in a 1% Tween\u201080 micellar medium at 370\u2009nm for itraconazole and 288\u2009nm for ibuprofen, following an excitation at 260\u2009nm for both drugs. The fluorescent intensity\u2013concentration relationships for both drugs were linear within the concentration ranges of 30\u2013400\u2009ng\u2009mL<jats:sup>\u22121<\/jats:sup> for itraconazole and 300\u20135000\u2009ng\u2009mL<jats:sup>\u22121<\/jats:sup> for ibuprofen, showing high correlation coefficient values. The method exhibited high precision, with quantitation limits of 13.3\u2009ng\u2009mL<jats:sup>\u22121<\/jats:sup> for itraconazole and 260.6\u2009ng\u2009mL<jats:sup>\u22121<\/jats:sup> for ibuprofen. Successful application to commercial tablet formulations demonstrated the method's accuracy and sensitivity. Validation per ICH guidelines confirmed its effectiveness for quantifying the drugs in spiked human plasma, achieving high recovery rates. Furthermore, an evaluation of the method's environmental impact using GAPI and analytical Eco\u2010Scale metrics confirmed its eco\u2010friendliness.<\/jats:p>","DOI":"10.1002\/bio.70071","type":"journal-article","created":{"date-parts":[[2025,1,2]],"date-time":"2025-01-02T21:19:43Z","timestamp":1735852783000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Simultaneous Eco\u2010Friendly Spectrofluorometric Estimation of Itraconazole and Ibuprofen in Pharmaceutical and Biological Matrices"],"prefix":"10.1002","volume":"40","author":[{"given":"Engy\u00a0Attaa","family":"Bakr","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Mansoura University  Mansoura Egypt"},{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy Horus University  New Damietta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5426-5993","authenticated-orcid":false,"given":"Abdallah\u00a0M.","family":"Zeid","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1468-1367","authenticated-orcid":false,"given":"Shereen\u00a0M.","family":"Shalan","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasser","family":"El\u2010Shabrawy","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/44.4.429"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2019.03.028"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mycmed.2018.03.002"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki264"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2009.00504.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.1.41-47.2004"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijdd.ijdd_20_17"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsos.210354"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/bio.3750"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2018.06.102"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/22297928.2016.1221776"},{"key":"e_1_2_8_13_1","first-page":"1","article-title":"Spectrophotometric Methods for the Determination of Ibuprofen in Tablets","volume":"18","author":"Wahbi A. A.","year":"2005","journal-title":"Pakistan Journal of Pharmaceutical Sciences"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2005.01.010"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826076.2012.678028"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/bmz031"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.4236\/ajac.2010.12007"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2003.08.031"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2006.03.024"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2004.11.014"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2006.04.007"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2003.10.036"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/02652030802383160"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2016.01.040"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.1.141-145.1999"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsos.200571"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/jccs.200700054"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1258\/0004563053492711"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.8.3569-3571.2005"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0934-8840(11)80719-X"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2018.01.004"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2018.01.013"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408347.2023.2276294"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2012.03.013"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/bio.4888"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13065-023-01001-5"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-9936(84)85006-2"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408349008051631"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/0731-7085(95)01530-X"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1081\/AL-120026416"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9797(86)90183-9"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2015.05.026"},{"key":"e_1_2_8_43_1","unstructured":"\u201cICH Harmonized Tripartite Guideline Validation of Analytical Procedures: Text and Methodology Q2(R1) Current Step 4 Version Parent Guidelines on Methodology Dated November 6 1996 Incorporated in November 2005.\u201d"},{"key":"e_1_2_8_44_1","volume-title":"Statistics and Chemometrics for Analytical Chemistry","author":"Miller J. N.","year":"2010"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2022.123742"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aej.2024.10.089"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10895-023-03175-6"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2023.341174"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2018.11.054"},{"key":"e_1_2_8_50_1","first-page":"864","article-title":"Combined Unfolded Principal Component Analysis and Artificial Neural Network for Determination of Ibuprofen in Human Serum by Three\u2010Dimensional Excitation\u2010Emission Matrix Fluorescence Spectroscopy","volume":"17","author":"Bahrami G.","year":"2018","journal-title":"Iran J Pharm Res"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2017.10.092"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10895-013-1276-9"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemolab.2010.05.012"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.59566\/IJBS.2009.5136"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1002\/bio.962"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-9140(03)00095-X"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(00)00584-7"}],"container-title":["Luminescence"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bio.70071","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T12:05:55Z","timestamp":1759925155000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bio.70071"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":56,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1002\/bio.70071"],"URL":"https:\/\/doi.org\/10.1002\/bio.70071","archive":["Portico"],"relation":{},"ISSN":["1522-7235","1522-7243"],"issn-type":[{"value":"1522-7235","type":"print"},{"value":"1522-7243","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"2024-10-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70071"}}