{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T10:44:44Z","timestamp":1792061084712,"version":"4.4.0"},"reference-count":46,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,2,16]],"date-time":"2025-02-16T00:00:00Z","timestamp":1739664000000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>This study explores the green synthesis of copper nanoparticles (Cu\u2010NPs) using Panchagavya, a traditional organic substance composed of five cow\u2010derived components, as a reducing and stabilizing agent. Cu\u2010NPs were characterized using UV\u2013Vis, FT\u2010IR, XRD, SEM\u2010EDS, TEM, DLS, and zeta potential analysis, which revealed their size, shape, and elemental properties. They exhibited strong antibacterial activity against <jats:styled-content style=\"fixed-case\"><jats:italic>Bacillus cereus<\/jats:italic><\/jats:styled-content> and <jats:styled-content style=\"fixed-case\"><jats:italic>Pseudomonas aeruginosa<\/jats:italic><\/jats:styled-content>. Biofilm inhibition was observed at various concentrations, with 38.98% at \u00bd MIC, 67.48% at MIC, and 84.03% at 2\u00d7 MIC. SEM analysis confirmed that Cu\u2010NPs disrupted the bacterial cell membrane, causing leakage of cellular contents. Antioxidant assays (DPPH, FRAP) revealed high scavenging activity, with percentages of 88.50% and 92.54%, respectively. Cu\u2010NPs showed anticancer activity on MCF7 cells, with an IC<jats:sub>50<\/jats:sub> of 38.18\u2009\u03bcg\/mL. Additionally, Cu\u2010NPs significantly reduced nitric oxide (NO) production in RAW 264.7 macrophage cells in a dose\u2010dependent manner. The Cu\u2010NPs also exhibited larvicidal efficacy, with 99.12% mortality against <jats:styled-content style=\"fixed-case\"><jats:italic>Aedes aegypti<\/jats:italic><\/jats:styled-content> and 95.26% against <jats:styled-content style=\"fixed-case\"><jats:italic>Culex quinquefasciatus<\/jats:italic><\/jats:styled-content>, and LC<jats:sub>50<\/jats:sub> values of 29.40\u2009\u03bcg\/mL and 93.55\u2009\u03bcg\/mL, respectively. Morphological changes in treated larvae included body shrinkage and degeneration of tracheal tube and ventral brush were noticed as compared to control. Histopathological examinations of Cu\u2010NP\u2010treated larvae showed several structural damages, including damage to gut epithelial cells, dissipation of the muscle layer, and loss of goblet cells. GC\u2013MS analysis of Panchagavya revealed its potential for various biological applications. These findings highlight the eco\u2010friendly and multifunctional nature of Panchagavya\u2010mediated Cu\u2010NPs, demonstrating their potential for antimicrobial, antioxidant, anticancer, and larvicidal applications, which could contribute to sustainable pest and disease management strategies.<\/jats:p>","DOI":"10.1002\/bio.70117","type":"journal-article","created":{"date-parts":[[2025,2,16]],"date-time":"2025-02-16T18:29:56Z","timestamp":1739730596000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Green Synthesis of Copper Nanoparticles Using Panchagavya: Nanomaterials for Antibacterial, Anticancer, and Environmental Applications"],"prefix":"10.1002","volume":"40","author":[{"given":"Samuthirapandi","family":"Muniasamy","sequence":"first","affiliation":[{"name":"Department of Microbiology Ayya Nadar Janaki Ammal College (Autonomous), Affiliated to Madurai Kamaraj University  Sivakasi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marissa","family":"Angelina","sequence":"additional","affiliation":[{"name":"Research Centre for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency (BRIN)  South Tangerang Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ponnirul","family":"Ponmanickam","sequence":"additional","affiliation":[{"name":"Department of Zoology Ayya Nadar Janaki Ammal College (Autonomous), Affiliated to Madurai Kamaraj University  Sivakasi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mysoon\u00a0M.","family":"Al\u2010Ansari","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antony","family":"Mythili","sequence":"additional","affiliation":[{"name":"School of Physics and Electronic Information Yan'an University  Yan'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saurav","family":"Dixit","sequence":"additional","affiliation":[{"name":"Centre of Research Impact and Outcome Chitkara University Institute of Engineering and Technology, Chitkara University  Rajpura Punjab India"},{"name":"Division of Research and Innovation Uttaranchal University  Dehradun India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5165-4272","authenticated-orcid":false,"given":"Krishnan","family":"Raguvaran","sequence":"additional","affiliation":[{"name":"Research Centre for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency (BRIN)  South Tangerang Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,16]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaim.2021.09.003"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-19-2639-6_16"},{"issue":"2","key":"e_1_2_13_4_1","first-page":"1","article-title":"Panchgavya: Pharmaco\u2010Therapeutic Perspectives","volume":"18","author":"Sadariya K. A.","year":"2019","journal-title":"Journal of Veterinary Pharmacology and Therapeutics"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.9734\/ijecc\/2024\/v14i54183"},{"issue":"4","key":"e_1_2_13_6_1","first-page":"165","article-title":"Therapeutic Potential of Panchagavya: A Review","volume":"9","author":"Patil S.","year":"2023","journal-title":"International Journal of Ayurvedic and Herbal Medicine"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crgsc.2021.100176"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ddr.21515"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202308413"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-08-102579-6.00017-4"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ddr.21726"},{"key":"e_1_2_13_12_1","first-page":"357","article-title":"Panchagavya\u2013a key Tool in Agriculture","author":"Ponmanickam P.","year":"2023","journal-title":"Advances in Bioscience and Biotechnology"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.susmat.2022.e00540"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matlet.2022.132000"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jambio\/lxad144"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-1378-8_9"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envres.2023.116698"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/1811199a0"},{"key":"e_1_2_13_19_1","first-page":"15","volume-title":"Methods in Enzymology","author":"Benzie I. F.","year":"1999"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2022.898324"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2024.103101"},{"key":"e_1_2_13_22_1","unstructured":"WHO \u201cGuidelines for Laboratory and Field Testing of Mosquito Larvicides \u201d World Heal Organ (2005) 1\u201341."},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s42690-021-00656-8"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jece.2022.107130"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-020-10127-1"},{"key":"e_1_2_13_26_1","first-page":"319","article-title":"X ray Diffraction (XRD) Analysis and Evaluation of Antioxidant Activity of Copper Oxide Nanoparticles Synthesized From Leaf Extract of Cissus Vitiginea","volume":"51","author":"Thakar M. A.","year":"2022","journal-title":"Maternit\u00e9"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.napere.2024.100091"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.inoche.2023.110448"},{"key":"e_1_2_13_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.triboint.2023.108891"},{"key":"e_1_2_13_30_1","doi-asserted-by":"publisher","DOI":"10.33263\/BRIAC121.12301236"},{"key":"e_1_2_13_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-015-1840-3"},{"key":"e_1_2_13_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0222322"},{"key":"e_1_2_13_33_1","doi-asserted-by":"publisher","DOI":"10.1080\/21691401.2020.1817053"},{"key":"e_1_2_13_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nano.2011.05.007"},{"key":"e_1_2_13_35_1","doi-asserted-by":"publisher","DOI":"10.1515\/ntrev-2016-0040"},{"key":"e_1_2_13_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envres.2023.116698"},{"key":"e_1_2_13_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00449-020-02357-x"},{"key":"e_1_2_13_38_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/7967294"},{"key":"e_1_2_13_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molstruc.2021.131437"},{"key":"e_1_2_13_40_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2023.1246728"},{"key":"e_1_2_13_41_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S466042"},{"key":"e_1_2_13_42_1","first-page":"3501","article-title":"Copper Nanoparticles Green Synthesis and Characterization as Anticancer Potential in Breast cancer Cells (MCF7) Derived From Prunus Nepalensis Phytochemicals","volume":"49","author":"Biresaw S. S.","year":"2022","journal-title":"Maternit\u00e9"},{"issue":"3","key":"e_1_2_13_43_1","first-page":"639","article-title":"Wound Healing Activity of Green Synthesized Copper Nanoparticles Through Cell Proliferation\u2010Migration, Antimicrobial Effects, and Nitric Oxide Triggering","volume":"79","author":"Hakimzadeh S.","year":"2024","journal-title":"Archives of Razi Institute"},{"key":"e_1_2_13_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.inoche.2024.112095"},{"key":"e_1_2_13_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10876-021-02151-z"},{"key":"e_1_2_13_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2018.11.022"},{"key":"e_1_2_13_47_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.01461"}],"container-title":["Luminescence"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bio.70117","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T13:36:21Z","timestamp":1759930581000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bio.70117"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2]]},"references-count":46,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,2]]}},"alternative-id":["10.1002\/bio.70117"],"URL":"https:\/\/doi.org\/10.1002\/bio.70117","archive":["Portico"],"relation":{},"ISSN":["1522-7235","1522-7243"],"issn-type":[{"value":"1522-7235","type":"print"},{"value":"1522-7243","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2]]},"assertion":[{"value":"2024-11-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70117"}}