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TC\u2010Fe<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub>\u2010NPs (<jats:italic>T. cordifolia<\/jats:italic>\u2010synthesized iron oxide nanoparticles) were characterized using UV\u2013Vis spectroscopy, FT\u2010IR, XRD, and FESEM\u2010EDAX. An absorption peak at 228\u2009nm confirmed the synthesis, while FT\u2010IR identified functional groups. XRD analysis verified the crystalline nature with prominent hkl planes at (220), (311), (400), (422), and (440). FESEM revealed a mean surface volume diameter of 82.21\u2009nm. Adsorption studies showed optimal conditions with 10\u2009ppm dye, 1\u2009mg of adsorbent, 60\u2009min of contact time, and pH\u20097. Under these conditions, the nanoparticles achieved a maximum adsorption efficiency of 88\u2009\u00b1\u20092.42% and a qmax of 104.49\u2009mg\u2009g<jats:sup>\u22121<\/jats:sup>, fitting the Langmuir isotherm model. Additionally, TC\u2010Fe<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub>\u2010NPs exhibited antioxidant activity with IC<jats:sub>50<\/jats:sub> values of 77.56\u2009\u00b1\u20094.69\u2009\u03bcg\/mL and 28.67\u2009\u00b1\u20092.59\u2009\u03bcg\/mL in the DPPH and ABTS assays, respectively. The adsorbed dye was efficiently desorbed, enabling reusability for up to 5\u2009cycles. Additionally, TC\u2010Fe<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub>\u2010NPs demonstrated antioxidant activity, reducing dye\u2010induced toxicity in <jats:styled-content style=\"fixed-case\"><jats:italic>Allium cepa<\/jats:italic><\/jats:styled-content> roots and non\u2010pathogenic bacteria, contributing to healthier root growth and enhanced bacterial viability. These findings highlight TC\u2010Fe<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub>\u2010NPs as efficient, reusable adsorbents with potential environmental applications.<\/jats:p>","DOI":"10.1002\/bio.70133","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T12:37:05Z","timestamp":1741696625000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Biogenic Fabrication of Iron Oxide Nanoparticles Using <i>Tinospora cordifolia<\/i> Leaf Extract: A Green Approach for Enhanced Antioxidant Activity and Bio Adsorption, Reusability, Toxicity Analysis of Methylene Blue Dye"],"prefix":"10.1002","volume":"40","author":[{"given":"Janani","family":"Ravi","sequence":"first","affiliation":[{"name":"Department of Biochemistry, School of Bioscience Periyar University  Salem Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subash\u00a0Chandra\u00a0Bose","family":"Ragunathan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Bioscience Periyar University  Salem Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santhiya","family":"Mani","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Bioscience Periyar University  Salem Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9753-0141","authenticated-orcid":false,"given":"R.","family":"Mythili","sequence":"additional","affiliation":[{"name":"Department of Biomaterials, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences Saveetha University  Chennai, Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saurav","family":"Dixit","sequence":"additional","affiliation":[{"name":"Centre of Research Impact and Outcome, Chitkara University Institute of Engineering and Technology Chitkara University  Rajpura Punjab India"},{"name":"Division of Research &amp; 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