{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T11:41:38Z","timestamp":1767008498782,"version":"build-2065373602"},"reference-count":16,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T00:00:00Z","timestamp":1741564800000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009392","name":"Prince Sattam bin Abdulaziz University","doi-asserted-by":"publisher","award":["PSAU\/2024\/R\/1445"],"award-info":[{"award-number":["PSAU\/2024\/R\/1445"]}],"id":[{"id":"10.13039\/100009392","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Larotrectinib or VITRAKVI is a first\u2010generation pan\u2013tropomyosin\u2010related kinase (TRK) inhibitor. It is a highly selective, tumor\u2010agnostic agent which has been approved in both adult and pediatric patients. Three different analytical methods for estimation of such important drug were developed. Two simple spectrophotometric methods were developed: Method I involves measuring the absorbance of different concentrations of the larotrectinib at 264\u2009nm in concentrations ranging from 2 to 15\u2009\u03bcg\/mL in ethanol while first derivative peak amplitude (Method II) was derived and measured at 278\u2009nm in concentration ranges from 2 to 15\u2009\u03bcg\/mL. A spectrofluorimetric technique was carried out by creating an ion pair complex through the interaction of larotrectinib with eosin Y in the presence of a Britton\u2013Robinson buffer at pH\u20093.5. The fluorescence quenching of the resulting complex was then detected at 535\u2009nm as the emission peak following excitation at 265\u2009nm. The spectrofluorimetric technique exhibited high sensitivity and linearity across concentration ranging between 0.5 and 10\u2009\u03bcg\/mL. These three methods were effectively utilized to quantify larotrectinib in both its raw form and its pharmaceutical formulation. Greenness of the developed methodologies was concerned and evaluated via three different tools including analytical ecoscale, green analytical procedure index (GAPI), and analytical greenness metric approach (AGREE).<\/jats:p>","DOI":"10.1002\/bio.70141","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T02:07:41Z","timestamp":1741745261000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Green and Sensitive Investigation for Estimation of Anticancer Medication in Raw Material and Dosage Form: Greenness Assessment Consideration"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9022-2632","authenticated-orcid":false,"given":"Samah\u00a0Abo","family":"El\u00a0Abass","sequence":"first","affiliation":[{"name":"Pharmaceutical Chemistry Department, College of Pharmacy Prince Sattam Bin Abdulaziz University  Al\u2010Kharj Kingdom of Saudi Arabia"},{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manal\u00a0A.","family":"Alossaim","sequence":"additional","affiliation":[{"name":"Pharmaceutical Chemistry Department, College of Pharmacy Prince Sattam Bin Abdulaziz University  Al\u2010Kharj Kingdom of Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taibah","family":"Aldakhil","sequence":"additional","affiliation":[{"name":"Pharmaceutical Chemistry Department, College of Pharmacy Prince Sattam Bin Abdulaziz University  Al\u2010Kharj Kingdom of Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alanoud\u00a0M.","family":"Aldawsari","sequence":"additional","affiliation":[{"name":"Pharmaceutical Chemistry Department, College of Pharmacy Prince Sattam Bin Abdulaziz University  Al\u2010Kharj Kingdom of Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fay\u00a0F.","family":"Alanzi","sequence":"additional","affiliation":[{"name":"Pharmaceutical Chemistry Department, College of Pharmacy Prince Sattam Bin Abdulaziz University  Al\u2010Kharj Kingdom of Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,3,10]]},"reference":[{"key":"e_1_2_14_2_1","unstructured":"U.S. Food and Drug Administration. Center for Drug Evaluation and Research \u201cFDA Approves Larotrectinib for Solid Tumors With NTRK Gene Fusions \u201d FDA; (2018) https:\/\/www.fda.gov\/drugs\/fda\u2010approves\u2010larotrectinib\u2010solid\u2010tumors\u2010ntrk\u2010gene\u2010fusions."},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj067"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S235934"},{"key":"e_1_2_14_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.4953"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2023.122914"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1752\u2010153X\u20105\u201360"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scp.2024.101493"},{"key":"e_1_2_14_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13065\u2010024\u201001137\u2010y"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/bio.3971"},{"key":"e_1_2_14_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2022.121919"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2012.03.013"},{"key":"e_1_2_14_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2018.01.013"},{"key":"e_1_2_14_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c01887"},{"key":"e_1_2_14_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2013.04.010"},{"key":"e_1_2_14_16_1","doi-asserted-by":"publisher","DOI":"10.1515\/revac\u20102017\u20130020"},{"volume-title":"Validation of Analytical Procedures: Text and Methodology Q2(R1)","year":"2005","author":"International Conference on Harmonization","key":"e_1_2_14_17_1"}],"container-title":["Luminescence"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bio.70141","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T18:43:41Z","timestamp":1759949021000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bio.70141"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3]]},"references-count":16,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["10.1002\/bio.70141"],"URL":"https:\/\/doi.org\/10.1002\/bio.70141","archive":["Portico"],"relation":{},"ISSN":["1522-7235","1522-7243"],"issn-type":[{"type":"print","value":"1522-7235"},{"type":"electronic","value":"1522-7243"}],"subject":[],"published":{"date-parts":[[2025,3]]},"assertion":[{"value":"2024-11-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70141"}}