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We achieved this by using the gas chromatography\u2013mass spectrometry (GC\u2010MS) method in polar solvents like methanol, personal ether and ethanol. We evaluated the crude sample in a variety of solvents and found several distinct chemicals, including phenolics, terpenoids, flavonoids, carbohydrates, amino acids, glycosides, proteins, steroids, tannins and alkaloids. According to the outcomes of the GC\u2010MS analysis, the mediums containing methanol, ethanol and pet ether each included 78, 82 and 38 chemical components, respectively. Sesquiterpenoids, phenolic compounds, amino substances and long\u2010chain hydrocarbons were the most abundant chemicals. The outcomes of the experiment, along with the presence of a variety of phytochemicals in this plant, encouraged us to carry out the microwave synthesis of silver nanoparticles from this leaf extract and analyse them. We used the MTT test on the A431 cell line, to assess the damaging effects of aqueous CT extracts and CT\u2010Ag NPs. Dual and DAPI staining confirmed the apoptotic activity of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Cajanus trinervius<\/jats:italic>\n                    <\/jats:styled-content>\n                    leaf extract along with their ROS release assessment confirmation by DCFDA staining.\n                  <\/jats:p>","DOI":"10.1002\/bio.70146","type":"journal-article","created":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T04:23:42Z","timestamp":1744259022000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Nuclear Staining and Fluorogenic Probe\u2013Based Evaluation of ROS\u2010Cytotoxic Potential Using AgNPs Synthesized From\n                    <scp>\n                      <i>Cajanus trinervius<\/i>\n                    <\/scp>\n                    Against A431 Cell Line"],"prefix":"10.1002","volume":"40","author":[{"given":"Jayanthi","family":"Kamalanathan","sequence":"first","affiliation":[{"name":"Department of Chemistry Government Arts College, Udhagamandalam  Nilgiris Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.\u00a0S.","family":"Suresh","sequence":"additional","affiliation":[{"name":"Department of Chemistry Government Arts College, Udhagamandalam  Nilgiris Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Venkatesh","family":"Rajendran","sequence":"additional","affiliation":[{"name":"Department of Biochemistry PG and Research Kongunadu Arts and Science College  Coimbatore Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamad\u00a0S.","family":"AlSalhi","sequence":"additional","affiliation":[{"name":"Department of Physics and Astronomy, College of Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandhanasamy","family":"Devanesan","sequence":"additional","affiliation":[{"name":"Bioproducts Research Chair, Department of Zoology, College of Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arumugam","family":"Suresh","sequence":"additional","affiliation":[{"name":"Central Research Laboratory, Meenakshi Medical College Hospital and Research Institute Meenakshi Academy of Higher Education and Research  Kanchipuram Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jerrine","family":"Joseph","sequence":"additional","affiliation":[{"name":"Catalenet Clinical Development &amp; 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