{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:25:20Z","timestamp":1791300320473,"version":"4.1.0"},"reference-count":79,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study introduces a novel sensitive and environment\u2010friendly spectrofluorimetric method for determination of an important muscle relaxant drug, tizanidine, with no need for using organic solvents or any pre\u2010derivatization steps. This strategy depends on tizanidine quenching effect on the native fluorescence of newly synthesized nitrogen\u2010doped carbon quantum dots (N\u2010CQDs) from\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Tetraclinis articulata<\/jats:italic>\n                    <\/jats:styled-content>\n                    extract, for the first time, using a microwave\u2010assisted synthetic strategy in only 4\u2009min. Full characterization of the synthesized N\u2010CQDs was performed by different microscopic and spectroscopic techniques. The synthesized N\u2010CQDs showed good fluorescence emission at 380\u2009nm when excited at 312\u2009nm. This method is the first one for tizanidine analysis spectrofluorimetrically using N\u2010CQDs as nanosensors. The developed approach demonstrated good linearity within the range of 0.1\u20131.0\u2009\u03bcg\/mL with a correlation coefficient of 0.9997 and a detection limit of 0.024\u2009\u03bcg\/mL. The developed approach was successfully applied for tizanidine estimation in its tablets and human urine samples with high % recoveries (98.04\u2013101.67) and low %RSD (&lt;\u20091.5) values. Furthermore, the content uniformity testing of the tizanidine tablets was performed in accordance with the USP guidelines. The method's eco\u2010friendliness was evaluated using AGREE and ComplexGAPI metrics. Validation of the method was performed according to ICHQ2 (R2) guidelines.\n                  <\/jats:p>","DOI":"10.1002\/bio.70160","type":"journal-article","created":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T01:02:39Z","timestamp":1743728559000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Rapid Microwave\u2010Assisted Synthesis of Nitrogen\u2010Doped Carbon Quantum Dots as Fluorescent Nanoprobe for Sensitive Estimation of Tizanidine in Human Urine and Dosage Forms: Application to Content Uniformity Testing"],"prefix":"10.1002","volume":"40","author":[{"given":"Sara","family":"Elhak","sequence":"first","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasser","family":"El\u2010Shabrawy","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fathalla","family":"Belal","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0543-5065","authenticated-orcid":false,"given":"Galal","family":"Magdy","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Kafrelsheikh University  Kafrelsheikh Egypt"},{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Mansoura National University  Gamasa Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,1]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2013.06.065"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcme.2016.06.006"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2017.12.037"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.977726"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtchem.2018.03.003"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2024.125067"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talo.2025.100427"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2023.122914"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2020.128539"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-32494-8"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scp.2024.101488"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scp.2023.101424"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/bio.4921"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1RA08452F"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28052351"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2025.113248"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612829666221103124856"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/bio.4643"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scp.2024.101691"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2024.124207"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talo.2025.100426"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13065-022-00894-y"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsos.220285"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2018.12.042"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1EN00017A"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2RA05759J"},{"key":"e_1_2_8_28_1","article-title":"Recent Developments in Carbon Quantum Dots: Properties, Fabrication Techniques, and Bio\u2010Applications","volume":"9","author":"El\u2010Shabasy R. 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