{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T16:19:15Z","timestamp":1783786755616,"version":"3.55.0"},"reference-count":28,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T00:00:00Z","timestamp":1745884800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Epinastine (EPS), an antihistaminic drug, is used to prevent itching of the eye caused by a condition known as allergic conjunctivitis. Few reported methods were reported for detecting EPS. Most of these reported methods are chromatographic methods that require expensive techniques and hazardous solvents. Inspired by EPS's medical importance and the absence of native fluorescence of ESP, we introduce a sensitive fluorimetric method for detecting EPS in eye drops and artificial aqueous humor for the first time. The method presented here utilizes a well\u2010known derivatization reaction called Hantzsch derivatization reaction to convert the nonfluorescent EPS drug to a fluorescent derivative. All the factors affecting the sensitivity of the developed method were optimized to enhance the method's sensitivity. After optimizations, this reaction enables the fluorimetric quantification of EPS at\n                    <jats:italic>\u03bb<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>em<\/jats:italic>\n                    <\/jats:sub>\n                    484\u2009nm (\n                    <jats:italic>\u03bb<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>ex<\/jats:italic>\n                    <\/jats:sub>\n                    \u2009=\u2009410\u2009nm) in eye drops and spiked aqueous humor without any interference from the excipients or naturally present biomolecules. The dynamic range for EPS detection in the pure state is 0.1\u20131.0\u2009\u03bcg\/mL (LOQ\u2009=\u200979.23\u2009ng\/mL and LOD\u2009=\u200926.15\u2009ng\/mL). The approach exhibits favorable percentage recoveries in spiked aqueous humor (95.80\u2009\u00b1\u20091.57% to 100.80\u2009\u00b1\u20091.89%).\n                  <\/jats:p>","DOI":"10.1002\/bio.70183","type":"journal-article","created":{"date-parts":[[2025,4,30]],"date-time":"2025-04-30T02:41:08Z","timestamp":1745980868000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["One\u2010Pot Synthesis of Hantzsch Reaction for Fluorometric Quantification of Epinastine in Aqueous Humor Samples"],"prefix":"10.1002","volume":"40","author":[{"given":"Abobakr\u00a0A.","family":"Mohamed","sequence":"first","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Fayoum University  Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed\u00a0Ibrahim","family":"Halawa","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Science United Arab Emirates University  Al Ain UAE"},{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmoud\u00a0A.","family":"Omar","sequence":"additional","affiliation":[{"name":"Analytical Chemistry Department, Faculty of Pharmacy Minia University  Minia Egypt"},{"name":"Department of Pharmacognosy and Pharmaceutical Chemistry, College of Pharmacy Taibah University  Al\u2010Madinah Al\u2010Munawwarah Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3703-7221","authenticated-orcid":false,"given":"Islam\u00a0M.","family":"Mostafa","sequence":"additional","affiliation":[{"name":"Analytical Chemistry Department, Faculty of Pharmacy Minia University  Minia Egypt"},{"name":"Analytical Chemistry Department, Faculty of Pharmacy Minia National University  New Minia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/jop.2023.0132"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anai.2014.07.007"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2125.2005.2250"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0149-2918(04)90004-5"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10384-024-01108-9"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.2332\/allergolint.54.565"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1517\/17425250903117284"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2006.07.012"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00213-007-0814-z"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(96)00094-1"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826070903526402"},{"key":"e_1_2_8_13_1","first-page":"1314","article-title":"A More Rapid, Simple and Sensitive HPLC\u2010MS\/MS Method for Determination of Epinastine in Human Plasma and Application to a Bioequivalence Study","volume":"35","author":"Shi L.","year":"2016","journal-title":"Latin American Journal of Pharmacy"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/jaoac\/90.5.1266"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2007.04.032"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10337-011-2108-1"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.measurement.2021.109068"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijoes.2023.100140"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/bio.3292"},{"key":"e_1_2_8_20_1","first-page":"111","article-title":"Spectrophotometric and Spectrofluorimetric Determination of Amlodipine Besilate and Doxazosin Mesilate in Bulk and in Dosage Forms via Hantzsch Reaction","volume":"3","author":"Ayad M.","year":"2012","journal-title":"Journal of Biomedical and Pharmaceutical Research"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4RA16650G"},{"key":"e_1_2_8_22_1","first-page":"147","article-title":"Application of Hantzsch Reaction as a New Method for Spectrofluorimetric Determination of Some Cephalosporins","volume":"7","author":"El\u2010Hamda M. A.","year":"2017","journal-title":"Journal of Applied Pharmaceutical Science"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2024.124372"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2024.124395"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfda.2018.05.009"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2022.920468"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2017.07.021"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/bio.3787"},{"key":"e_1_2_8_29_1","unstructured":"ICH Harmonized tripartite Guideline Q2 (R1) \u201cValidation of Analytical Procedures. Text and Methodology Current Step 4 Versions Parent Guideline on Methodology Dated November 1996 Incorporated in November 2005.\u201d"}],"container-title":["Luminescence"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bio.70183","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,30]],"date-time":"2025-11-30T05:45:17Z","timestamp":1764481517000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bio.70183"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,29]]},"references-count":28,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.1002\/bio.70183"],"URL":"https:\/\/doi.org\/10.1002\/bio.70183","archive":["Portico"],"relation":{},"ISSN":["1522-7235","1522-7243"],"issn-type":[{"value":"1522-7235","type":"print"},{"value":"1522-7243","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,29]]},"assertion":[{"value":"2025-01-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70183"}}