{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T00:11:02Z","timestamp":1784247062876,"version":"3.55.0"},"reference-count":46,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,5,29]],"date-time":"2025-05-29T00:00:00Z","timestamp":1748476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Relevant studies have demonstrated that \u03b1\u2010glucosidase represents a crucial target for diabetes treatment, and andrographolide (AO) exhibits notable inhibitory activity against this enzyme. In this research, the interaction mechanism between AO and \u03b1\u2010glucosidase was investigated using UV\u2013Vis, fluorescence analysis, FT\u2010IR, circular dichroism (CD), and molecular docking techniques. AO induced static quenching of the fluorescent groups in \u03b1\u2010glucosidase through a spontaneous reaction process (\u0394\n                    <jats:italic>G<\/jats:italic>\n                    <jats:sup>0<\/jats:sup>\n                    \u2009&lt;\u20090), primarily driven by hydrogen bonds and van der Waals forces at a single binding site. The interaction between AO and \u03b1\u2010glucosidase resulted in a reduction of the \u03b1\u2010helix content while increasing the \u03b2\u2010turn and random coil components within the secondary structure of \u03b1\u2010glucosidase. A detailed analysis of molecular docking results revealed that AO interacted with Tyr158 and Tyr316 residues in \u03b1\u2010glucosidase, leading to fluorescence quenching of the enzyme and enhancing the hydrophobicity surrounding these tyrosine residues. Furthermore, AO formed hydrogen bonds with Glu277, Arg315, Asn415, and Arg442 while generating van der Waals interactions along with hydrophobic forces involving Phe159 and other amino acid residues. Through these intermolecular interactions, AO bound to the active center's binding cavity in \u03b1\u2010glucosidase, resulting in conformational changes that ultimately affect enzymatic activity.\n                  <\/jats:p>","DOI":"10.1002\/bio.70206","type":"journal-article","created":{"date-parts":[[2025,5,29]],"date-time":"2025-05-29T06:09:44Z","timestamp":1748498984000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Interaction Mechanism Between Andrographolide and \u03b1\u2010Glucosidase: Multispectroscopy and Molecular Docking Analyses"],"prefix":"10.1002","volume":"40","author":[{"given":"Nian\u2010Min","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Materials Science Hebei University  Baoding China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4497-0693","authenticated-orcid":false,"given":"Lei","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Nursing Hebei University  Baoding China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan\u2010Bing","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Materials Science Hebei University  Baoding China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Long","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Materials Science Hebei University  Baoding China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li\u2010Qing","family":"Yu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Materials Science Hebei University  Baoding China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi\u2010Gang","family":"Shen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Materials Science Hebei University  Baoding China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun\u2010Kai","family":"Lv","sequence":"additional","affiliation":[{"name":"Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Materials Science Hebei University  Baoding China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,29]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1055\/a\u20101018\u20109078"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-71908-9"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.21276\/ap.2021.10.1.12"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2023.115119"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2018.1501657"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.18388\/abp.2008_3087"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00044-020-02612-6"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2021.129837"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2016.11.089"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/bio.4444"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jlumin.2024.120437"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2020.126346"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/00387010.2024.2313788"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2015.05.088"},{"key":"e_1_2_10_16_1","first-page":"1","article-title":"Inhibitory Mechanism of Chrysin and Diosmetinto \u03b1\u2010Glucosidase: Insights From Kinetics, Multispectroscopy and Molecular Docking Investigations","volume":"30","author":"Zhang Y.","year":"2024","journal-title":"Journal of Biomolecular Structure & Dynamics"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2022.114179"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1RA07753H"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2019.12.021"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2018.07.148"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arabjc.2024.105802"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/bio.4384"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5FO00992H"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods12102054"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11947-024-03372-6"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fochx.2023.100986"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/fqsafe\/fyad064"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.129499"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.129573"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.130535"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.4c00699"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2024.107177"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbio.2023.102680"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2022.04.035"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molstruc.2023.136176"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2019.103587"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodhyd.2023.108547"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2008.07.010"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2024.1425832"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.inoche.2024.112586"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1FO01042E"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11694-021-00920-y"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2023.105648"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph15020205"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molstruc.2023.135567"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6FO00680A"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.130982"}],"container-title":["Luminescence"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bio.70206","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,30]],"date-time":"2025-11-30T06:02:43Z","timestamp":1764482563000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bio.70206"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,29]]},"references-count":46,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,6]]}},"alternative-id":["10.1002\/bio.70206"],"URL":"https:\/\/doi.org\/10.1002\/bio.70206","archive":["Portico"],"relation":{},"ISSN":["1522-7235","1522-7243"],"issn-type":[{"value":"1522-7235","type":"print"},{"value":"1522-7243","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,29]]},"assertion":[{"value":"2025-03-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70206"}}