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Spectroscopic analyses reveal that OLTN significantly attenuates the intrinsical fluorescence of BSA through the formation of an OLTN\u2013BSA complex, indicating a static quenching mechanism. Experimental data further confirmed the formation of a nonfluorescent OLTN\u2013BSA complex. These findings provide valuable insights into the molecular interactions governing the OLTN\u2013BSA system, enhancing our understanding of drug\u2013protein binding dynamics. The binding constant and stoichiometric ratio of the complex were determined to be approximately 10\n                    <jats:sup>4<\/jats:sup>\n                    \u2009M\n                    <jats:sup>\u22121<\/jats:sup>\n                    and 1:1, respectively, suggesting moderate binding affinity of OLTN for BSA. Molecular docking assays verified that OLTN primarily associated with the Sudlow's Site II, inducing conformational alterations in both the secondary structure and quaternary structure of BSA. Thermodynamic and molecular docking analyses established that hydrophobicity interactions and hydrogen bonding (including conventional hydrogen bonding and the carbon hydrogen bonding) serve as the dominant driving forces for stabilizing the complex. This investigation provides critical guidance for understanding the pharmacodynamics and pharmacokinetics of OLTN in human, particularly regarding its plasma protein binding behavior and potential drug\u2013drug interactions.\n                  <\/jats:p>","DOI":"10.1002\/bio.70244","type":"journal-article","created":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T23:22:29Z","timestamp":1750807349000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Elucidating the Binding Mechanism of Oclacitinib With Bovine Serum Albumin: A Multidisciplinary Approach Combining Spectroscopy, Thermodynamics, and Molecular Docking"],"prefix":"10.1002","volume":"40","author":[{"given":"Dong","family":"Chen","sequence":"first","affiliation":[{"name":"College of Pharmaceutic Science Zhejiang University of Technology  Hangzhou 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