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TFP was determined using a simple, green, sensitive, and non\u2010extractive spectrofluorimetric approach in bulk and pharmaceutical formulation. The basic principle of this technique is the association complex that could be formed between TFP and erythrosine B in an acidic environment. The complex formation quenched the intrinsic erythrosine B fluorescence that was monitored at 552\u2009nm following excitation at 530\u2009nm. The linear concentration range was 50\u20131400\u2009ng\u2009mL<jats:sup>\u22121<\/jats:sup> with a respectable correlation coefficient (<jats:italic>r<\/jats:italic>\u2009=\u20090.9998). All factors influencing the production of the complex were investigated and adjusted. The LOD and LOQ were 15 and 46\u2009ng\u2009mL<jats:sup>\u22121<\/jats:sup>, respectively. For the validation criteria, the International Council on Harmonization (ICH) guidelines had been followed. It was possible to analyze TFP in bulk, tablet formulations, and evaluation of Stellasil tablet content uniformity using the proposed method with accurate, reproducible, and repeatable results. Finally, the greenness of the developed approach was assessed utilizing three advanced tools: the Eco\u2010Scale, GAPI, and AGREE. In addition, the blueness of the approach was evaluated using a new tool called BAGI.<\/jats:p>","DOI":"10.1002\/bio.70252","type":"journal-article","created":{"date-parts":[[2025,7,5]],"date-time":"2025-07-05T06:33:12Z","timestamp":1751697192000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Trifluoperazine Nano\u2010Level Assay in Pharmaceutical Formulation Using a Green and Simple Spectrofluorometric Approach Based on Ion Pairing With Erythrosine B: Application to Content Uniformity With Greenness and Blueness Evaluation"],"prefix":"10.1002","volume":"40","author":[{"given":"Khalid\u00a0M.","family":"Badr\u00a0El\u2010Din","sequence":"first","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Pharmacy Minia University  Minia Egypt"},{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Minia National University  New Minia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayed\u00a0M.","family":"Derayea","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Pharmacy Minia University  Minia Egypt"},{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Minia National University  New Minia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5965-7387","authenticated-orcid":false,"given":"Mohamed","family":"Oraby","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Sohag University  Sohag Egypt"},{"name":"Pharmaceutical Chemistry Department, College of Pharmacy Al\u2010Esraa University  Baghdad Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7625-4028","authenticated-orcid":false,"given":"Hadeer\u00a0A.","family":"Elhamdy","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Sohag University  Sohag Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-044452166-8\/50006-6"},{"key":"e_1_2_8_3_1","first-page":"123","article-title":"Simultaneous Determination of Chlorpromazine and Trifluoperazine in Pharmaceutical Preparations Using High Performance Liquid Chromatography","volume":"22","author":"Dhabab J. 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