{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:32:04Z","timestamp":1786977124115,"version":"build-2736575974"},"reference-count":43,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,24]],"date-time":"2025-07-24T00:00:00Z","timestamp":1753315200000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    It was the first time that a spectrofluorimetric approach for the simultaneous analysis of methotrexate (MET) and 6\u2010mercaptopurine (6\u2010MP) had been achieved. This study aims to propose an accurate and sensitive second derivative synchronous spectrofluorimetric approach for the measurement of MET and 6\u2010MP in different matrices simultaneously without the need for prior separation. The proposed approach measured the synchronous fluorescence intensity of pharmaceuticals under research at a constant wavelength difference (\u0394\u03bb)\u2009=\u2009130\u2009nm. For the quantitative analysis of 6\u2010MP and MET, the second derivative's peak amplitudes were observed at 380 and 400\u2009nm, respectively. The process was fully validated and showed exceptional linearity for either 6\u2010MP or MET in the concentration range of 10\u2013100\u2009ng\u2009mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    . The new method was used to quantitatively analyze both drugs in a synthetically formulated mixture, dosage forms, and spiked human plasma. A statistical comparison of the results with other published analytical techniques revealed no significant difference. The validation of the procedure was successfully completed in compliance with ICH guidelines. To assess the analytical methodology's greenness, Eco\u2010Scale and GAPI greenness tools were employed.\n                  <\/jats:p>","DOI":"10.1002\/bio.70265","type":"journal-article","created":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T02:39:22Z","timestamp":1753411162000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Green Synchronous Spectrofluorimetric Analysis of Methotrxate, With 6\u2010Mercaptopurine as Add\u2010On Therapy of Childhood Acute Lymphoblastic Leukemia: Application in Laboratory Prepared Mixtures, Spiked Human Plasma, and Dosage Forms"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6467-849X","authenticated-orcid":false,"given":"Hesham","family":"Salem","sequence":"first","affiliation":[{"name":"Pharmaceutical Chemistry Department, Faculty of Pharmacy Deraya University  New Minia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Amir","sequence":"additional","affiliation":[{"name":"Pharmaceutical Chemistry Department, Faculty of Pharmacy Deraya University  New Minia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omar","family":"Saied","sequence":"additional","affiliation":[{"name":"Pharmaceutical Chemistry Department, Faculty of Pharmacy Deraya University  New Minia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asmaa\u00a0A.","family":"Hamed","sequence":"additional","affiliation":[{"name":"Clinical Pharmacy Department, Faculty of Pharmacy New Valley University  New Valley Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maemona","family":"Mamdouh","sequence":"additional","affiliation":[{"name":"Pharmaceutical Chemistry Department, Faculty of Pharmacy Deraya University  New Minia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noha","family":"Khalid","sequence":"additional","affiliation":[{"name":"Pharmaceutical Chemistry Department, Faculty of Pharmacy Deraya University  New Minia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadin","family":"Mohasen","sequence":"additional","affiliation":[{"name":"Pharmaceutical Chemistry Department, Faculty of Pharmacy Deraya University  New Minia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gehad","family":"Alaswad","sequence":"additional","affiliation":[{"name":"Sednawi Children's Hospital, Zagazig University  Zagazig Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmoud","family":"Abdelgalil","sequence":"additional","affiliation":[{"name":"Pharmaceutical Chemistry Department, Faculty of Pharmacy Deraya University  New Minia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramy\u00a0M.","family":"Elsabaa","sequence":"additional","affiliation":[{"name":"Clinical Pharmacy Department, Faculty of Pharmacy Menoufia University  Shibin El Kom Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,24]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtice.2017.05.031"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(02)00952-2"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.11.037"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2015.08.017"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2016.10.035"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826076.2015.1087409"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3109\/03602538508991433"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-3623(99)00022-1"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.seminhematol.2008.09.007"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.24928"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826076.2017.1399417"},{"key":"e_1_2_11_13_1","first-page":"1373","article-title":"Development and Validation of HPLC Method to Determination of Methotrexate in Children Oncologic Patients","volume":"19","author":"Abreu C. 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