{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T20:54:00Z","timestamp":1790974440227,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T00:00:00Z","timestamp":1766016000000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100020970","name":"Rashtriya Uchchatar Shiksha Abhiyan","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100020970","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Prostate\u2010specific antigen (PSA) serves as a critical biomarker for the early diagnosis and monitoring of prostate malignancies. In this work, we report the fabrication of a carboxy\u2010functionalized porous organic polymer (POP) as a highly sensitive and selective fluorescent immunosensor for PSA detection. The POP was synthesized via a solvothermal Schiff base condensation, which bears free \u2013COOH functionalities to facilitate covalent antibody immobilization through classical EDC\/NHS coupling chemistry. The resulting POP\u2013antibody bioconjugate exhibited a pronounced fluorescence quenching response upon PSA binding, attributed to static quenching via ground\u2010state complex formation, as confirmed by steady\u2010state and time\u2010resolved photoluminescence studies. Under optimized conditions, the sensor achieved a limit of detection (LOD) of 14\u2009pg\/mL, demonstrating a wide linear dynamic range and minimal cross\u2010reactivity with nontarget biomolecules. The high surface area and hierarchical porosity of the POP matrix contributed to enhanced probe\u2013analyte interactions and signal amplification. Notably, the sensor retained excellent performance in complex matrices, with successful PSA quantification in artificial urine and blood samples, highlighting its potential for real\u2010world clinical diagnostics. The synergistic integration of structural surface functionality and photostability in this POP\u2010based platform underscores its promise for ultrasensitive, low\u2010cost, and point\u2010of\u2010care biosensing of cancer biomarkers.<\/jats:p>","DOI":"10.1002\/bio.70385","type":"journal-article","created":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T05:17:37Z","timestamp":1766121457000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Carboxylic Acid\u2013Functionalized \u03b2\u2010Ketoenamine Linked Porous Organic Polymer Based Fluorescent Immunoassay for Prostate Cancer Detection"],"prefix":"10.1002","volume":"40","author":[{"given":"Sreelakshmi\u00a0Thaniparambil","family":"Prasad","sequence":"first","affiliation":[{"name":"Department of Applied Chemistry Cochin University of Science and Technology  Kochi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1263-8827","authenticated-orcid":false,"given":"Anna\u00a0Merin","family":"Joy","sequence":"additional","affiliation":[{"name":"Department of Applied Chemistry Cochin University of Science and Technology  Kochi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7701-2793","authenticated-orcid":false,"given":"Kala","family":"Ramakrishnan","sequence":"additional","affiliation":[{"name":"Department of Applied Chemistry Cochin University of Science and Technology  Kochi India"},{"name":"Inter University Centre for Nanomaterials and Devices Cochin University of Science and Technology  Kochi India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,18]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2014.04.060"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncponc1187"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1041"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac2030199"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.6b04630.Point\u2010of\u2010Care"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1039\/d4sd00119b"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2011.10.007"},{"key":"e_1_2_11_9_1","first-page":"72","article-title":"Characterization of Monoclonal and Polyclonal Antibodies Recognizing Prostate Specific Antigen: Implication for Design of a Sandwich ELISA","volume":"11","author":"Mohseni S. R.","year":"2019","journal-title":"Avicenna Journal Of Medical Biotechnology"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2015.02.026"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2007.11.003"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2007.01.063"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13206\u2010019\u20104204\u20105"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2022.133269"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biosx.2022.100130"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2020.128994"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.9b00204"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.8b08312"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1039\/d4ra07875f"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcis.2017.09.009"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssuschemeng.7b02234"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsapm.3c03233"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1039\/c6ta11226a"},{"key":"e_1_2_11_24_1","unstructured":"M.S.E.Jagst 2011Using Chemical Derivatization X\u2010Ray Photoelectron Spectroscopy (CD\u2010XPS)."},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1039\/c7ta01438d"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsaem.0c02102"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2022.140154"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/01496395.2020.1820523"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-46312-4"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2015.08.043"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.4c00862"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.3c04078"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1088\/1361\u20106528\/ab36c9"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201019733\u2010z"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/6209731"}],"container-title":["Luminescence"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bio.70385","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T09:24:59Z","timestamp":1767000299000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bio.70385"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":34,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1002\/bio.70385"],"URL":"https:\/\/doi.org\/10.1002\/bio.70385","archive":["Portico"],"relation":{},"ISSN":["1522-7235","1522-7243"],"issn-type":[{"value":"1522-7235","type":"print"},{"value":"1522-7243","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"2025-09-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70385"}}