{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T16:01:03Z","timestamp":1776873663401,"version":"3.51.2"},"reference-count":13,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T00:00:00Z","timestamp":1766448000000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A sustainable and highly sensitive spectrofluorimetric method was developed for the determination of ceftobiprole medocaril, a fifth\u2010generation cephalosporin, in pharmaceutical dosage form and rat plasma samples. This approach utilizes carbon quantum dots functionalized through ion\u2010pair formation with ceftobiprole and tetraphenylborate, creating a selective fluorescent probe. Quantification is based on a measurable decrease in fluorescence intensity at 450\u2009nm following excitation at 365\u2009nm, resulting from the interaction between the drug and the probe. Method parameters were systematically optimized and validated according to ICH guidelines, demonstrating excellent linearity (0.25\u20132.00\u2009\u03bcg\/mL), sensitivity (LOD\u2009=\u20090.07\u2009\u03bcg\/mL), and precision. The method was successfully applied to pharmacokinetic analysis in Wistar rats following a single 50\u2010mg\/kg intravenous infusion over 5\u2009min. Pharmacokinetic parameters including C\n                    <jats:sub>max<\/jats:sub>\n                    (45\u2009\u03bcg\/mL), T\n                    <jats:sub>max<\/jats:sub>\n                    (0.25\u2009h), half\u2010life (3.17\u2009h), and systemic clearance (0.45\u2009L\/h\/kg) were obtained using non\u2010compartmental analysis. Greenness evaluation using AGREE and GAPI tools confirmed the method's minimal environmental impact. The results affirm the method's applicability for routine quality control, therapeutic monitoring, and preclinical pharmacokinetic studies.\n                  <\/jats:p>","DOI":"10.1002\/bio.70393","type":"journal-article","created":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T16:18:36Z","timestamp":1766506716000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Eco\u2010Friendly Fluorescent Probe\u2010Based Quantification of Ceftobiprole Medocaril: Greenness Assessment and Pharmacokinetic Study in Rats"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1794-9859","authenticated-orcid":false,"given":"Sherif","family":"Ramzy","sequence":"first","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Al\u2010Azhar University  Nasr City Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manal\u00a0E.","family":"Alosaimi","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences, College of Medicine Princess Nourah bint Abdulrahman University  Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maram\u00a0H.","family":"Abduljabbar","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, College of Pharmacy Taif University  Taif Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reem\u00a0M.","family":"Alnemari","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics and Pharmaceutical Technology, College of Pharmacy Taif University  Taif Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eid","family":"Alatwi","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, College of Pharmacy Jouf University  Sakaka Al\u2010Jouf Region Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atiah\u00a0H.","family":"Almalki","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, College of Pharmacy Taif University  Taif Saudi Arabia"},{"name":"Addiction and Neuroscience Research Unit, Health Science Campus Taif University  Taif Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,23]]},"reference":[{"key":"e_1_2_10_2_1","first-page":"3","article-title":"Mechanisms of Action and Antimicrobial Activity of Ceftobiprole","volume":"32","author":"Morosini M. I.","year":"2019","journal-title":"Revista Espa\u00f1ola de Quimioterapia"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics10020170"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/10600280241293773"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/aac.0051519"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2024.119806"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232315252"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/pr10112196"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/pr9040708"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2021-0423"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2025.114886"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/bio.4057"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2018.01.013"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c01887"}],"container-title":["Luminescence"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bio.70393","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T09:25:17Z","timestamp":1767000317000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bio.70393"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":13,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1002\/bio.70393"],"URL":"https:\/\/doi.org\/10.1002\/bio.70393","archive":["Portico"],"relation":{},"ISSN":["1522-7235","1522-7243"],"issn-type":[{"value":"1522-7235","type":"print"},{"value":"1522-7243","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"2025-08-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70393"}}