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Copper nanoclusters (CuNCs), leveraging Earth\u2010abundance, low expense, and noble metal\u2010like catalytic properties, offer an ideal nanozyme platform. This study harnesses the intrinsic peroxidase mimicking activity of CuNCs to construct a visual colorimetric sensor for ACP detection. CuNCs oxidize the substrate 3,3\u2032,5,5\u2032\u2010tetramethylbenzidine (TMB) to generate a blue chromogen (\u03bbmax\u2009=\u2009654\u2009nm). ACP introduction triggers reduction of this chromogen, diminishing both color intensity and absorbance. The optimized sensor achieves a linear detection range of 0\u20136\u2009U\/L with a detection limit of 0.027\u2009U\/L, demonstrating high sensitivity and specificity. This CuNCs\u2010based platform provides a rapid, low\u2010cost, and operationally simple approach for ACP quantification, highlighting significant potential for clinical diagnostics.<\/jats:p>","DOI":"10.1002\/bio.70396","type":"journal-article","created":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T15:19:30Z","timestamp":1766503170000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Colorimetric Detection of Acid Phosphatase Using Nanoparticle CuNCs as Peroxidase Mimicking Activity"],"prefix":"10.1002","volume":"40","author":[{"given":"Jinhua","family":"Fu","sequence":"first","affiliation":[{"name":"Department of Pharmacy Longyan First Hospital Affiliated to Fujian Medical University  Longyan Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gengneng","family":"Lai","sequence":"additional","affiliation":[{"name":"Institute of Materia Medica, School of Pharmacy, Fujian Provincial 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