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However, on\u2010site, rapid detection of these dual targets in complex water matrices remains a challenge. In this work, a nitrogen\u2010doped carbon dots (N\u2010CDs)\u2010Eu\n                    <jats:sup>3+<\/jats:sup>\n                    \u2010tetracycline (TC)\u2010based ratiometric fluorescence probe was constructed for turn \u201coff\u2013on\u201d determination of PPi and ALP based on the strong binding interaction between PPi and Eu\n                    <jats:sup>3+<\/jats:sup>\n                    and PPi hydrolyzed by ALP. In the N\u2010CDs\u2010Eu\n                    <jats:sup>\n                      <jats:bold>3<\/jats:bold>\n                      +\n                    <\/jats:sup>\n                    \u2010TC system, with the addition of PPi, PPi could replace the N\u2010CDs to form the PPi\u2010Eu\n                    <jats:sup>3+<\/jats:sup>\n                    \u2010TC multicomplex, and N\u2010CDs were released, resulting in the fluorescence quenching of Eu\n                    <jats:sup>3+<\/jats:sup>\n                    and fluorescence recovery of N\u2010CDs. ALP could hydrolyze PPi to PO\n                    <jats:sub>4<\/jats:sub>\n                    <jats:sup>3\u2212<\/jats:sup>\n                    , which could destroy the PPi\u2010Eu\n                    <jats:sup>3+<\/jats:sup>\n                    \u2010TC multicomplex and form the N\u2010CDs\u2010Eu\n                    <jats:sup>3+<\/jats:sup>\n                    \u2010TC multicomplex again, and the fluorescence of Eu\n                    <jats:sup>3+<\/jats:sup>\n                    at 617\u2009nm was recovered. The N\u2010CDs\u2010Eu\n                    <jats:sup>3+<\/jats:sup>\n                    \u2010TC\u2010based ratiometric fluorescent probe possesses a quick fluorescence response, obvious fluorescence color variance, and excellent selectivity and sensitivity with LOD of 40\u2009nM for PPi and 0.03\u2009U\/L for ALP. The proposed probe was successfully applied for the detection of PPi and ALP in the actual environmental water samples or serum samples.\n                  <\/jats:p>","DOI":"10.1002\/bio.70417","type":"journal-article","created":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T11:33:41Z","timestamp":1767872021000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Nitrogen\u2010Doped Carbon Dots\u2010Eu\n                    <sup>3+<\/sup>\n                    \u2010Tetracycline as a Ratiometric Fluorescence Probe for Turn \u201cOff\u2013On\u201d Determination of Pyrophosphate and Alkaline Phosphatase Activity"],"prefix":"10.1002","volume":"41","author":[{"given":"Shengnan","family":"Yin","sequence":"first","affiliation":[{"name":"State Key Laboratory of Soil Pollution Control and Safety Zhejiang University  Hangzhou China"},{"name":"Key Laboratory of Environmental Remediation and Ecological Health, Ministry of 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