{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T19:16:51Z","timestamp":1790795811294,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T00:00:00Z","timestamp":1772668800000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22507091"],"award-info":[{"award-number":["22507091"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Fibroblast activation protein\u2010\u03b1 (FAP\u2010\u03b1) is a crucial biomarker for pancreatic cancer, yet developing activatable near\u2010infrared (NIR) fluorescent probes for its real\u2010time imaging faces challenges in achieving high signal\u2010to\u2010background ratios and specificity. Here, we present\n                    <jats:bold>ZGP\u2010D<\/jats:bold>\n                    , a novel FAP\u2010\u03b1\u2010activated NIR probe designed to overcome these limitations.\n                    <jats:bold>ZGP\u2010D<\/jats:bold>\n                    integrates a DDAO fluorophore with a FAP\u2010\u03b1\u2010specific Cbz\u2013Gly\u2013Pro peptide, creating an efficient intramolecular charge transfer (ICT) quenching mechanism that ensures a minimal background signal. Upon specific enzymatic cleavage by FAP\u2010\u03b1, the probe exhibits a robust &gt;\u200940\u2010fold fluorescence turn\u2010on at 657\u2009nm, demonstrating exceptional sensitivity with a detection limit of 0.024\u2009\u03bcg\/mL and superior selectivity over various biological interferents.\n                    <jats:bold>ZGP\u2010D<\/jats:bold>\n                    successfully visualized endogenous FAP\u2010\u03b1 in living cells, clearly distinguishing high\u2010expression pancreatic cancer cells (BXPC\u20103) from low\u2010expression cells. Furthermore, in a BXPC\u20103 xenograft mouse model, local administration of\n                    <jats:bold>ZGP\u2010D<\/jats:bold>\n                    enabled high\u2010contrast tumor imaging, achieving a tumor\u2010to\u2010normal tissue signal ratio of 4.2. This work establishes\n                    <jats:bold>ZGP\u2010D<\/jats:bold>\n                    as a highly sensitive and specific molecular tool for monitoring FAP\u2010\u03b1 activity, highlighting its significant potential for advancing cancer diagnostics and fluorescence\u2010guided surgery.\n                  <\/jats:p>","DOI":"10.1002\/bio.70430","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T20:14:14Z","timestamp":1773000854000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A Near\u2010Infrared Off\u2013On Fluorescent Probe for Sensitive and Specific Imaging of Fibroblast Activation Protein\u2010\u03b1 Activity in Pancreatic Cancer"],"prefix":"10.1002","volume":"41","author":[{"given":"Jiao","family":"Xu","sequence":"first","affiliation":[{"name":"Wenzhou Institute University of Chinese Academy of Sciences  Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutong","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life and Environmental Science Wenzhou University  Wenzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhipeng","family":"Li","sequence":"additional","affiliation":[{"name":"School of Ophthalmology Optometry, School of Biomedical Engineering Wenzhou Medical University  Wenzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4351-4872","authenticated-orcid":false,"given":"Jianliang","family":"Shen","sequence":"additional","affiliation":[{"name":"Wenzhou Institute University of Chinese Academy of Sciences  Wenzhou China"},{"name":"School of Ophthalmology Optometry, School of Biomedical Engineering Wenzhou Medical University  Wenzhou Zhejiang 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