{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T01:09:13Z","timestamp":1773018553167,"version":"3.50.1"},"reference-count":59,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T00:00:00Z","timestamp":1771632000000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Soy protein isolate (SPI) enhances the functionality of flavonols, with their interaction and bioactivity critically dependent on B\u2010ring substitution. Multispectral analysis and molecular docking were used to investigate the interaction between SPI and flavonols (Kaempferide (Kaem), Galangin (Gal), Kaempferol (Kae), Myricetin (Myr)) and their resulting antiseptic\/antifungal performance. The results indicated that hydroxylation on the B\u2010ring of flavonols significantly enhanced their binding affinity to SPI through static quenching, primarily driven by hydrogen bonds and van der Waals forces, whereas methylation reduced it. Consequently, the binding affinity followed the order of Myr, Kae, Gal, and Kaem. Key functional findings demonstrated a structure\u2013activity relationship: The hydroxyl or methoxy group in B\u2010ring substantially enhanced antibacterial activity in the determination of colony counts. However, for antifungal activity against\n                    <jats:italic>Gibberella zeae<\/jats:italic>\n                    (\n                    <jats:italic>GZ<\/jats:italic>\n                    ) and\n                    <jats:italic>Physalospora piricola<\/jats:italic>\n                    (\n                    <jats:italic>PP<\/jats:italic>\n                    ), B\u2010ring hydroxylation was unfavorable, whereas methylation was beneficial. Consequently, Gal shows the best antifungal ability, followed by Kaem, Kae, and Myr. A notable discovery was that while pure flavonols or SPI alone promoted\n                    <jats:italic>GZ<\/jats:italic>\n                    growth, their formed flavonol\u2010SPI complexes reversed this effect, exerting inhibitory activity. These insights provide a significant reference for designing natural, green preservative systems by strategically combining specific flavonols with protein carriers like SPI.\n                  <\/jats:p>","DOI":"10.1002\/bio.70451","type":"journal-article","created":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T08:57:46Z","timestamp":1771664266000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of Hydroxyl or Methoxy Group in B\u2010Ring of Flavonols on Structural Change and Antiseptic Ability of Soy Protein Isolate"],"prefix":"10.1002","volume":"41","author":[{"given":"Mengyao","family":"Guo","sequence":"first","affiliation":[{"name":"School of Chemistry and Chemical Engineering Liaocheng University  Liaocheng Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinru","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical 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